FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
HSPC300, kug
FlyBase ID
FBig0000104537
Interaction Network
Interactions Browser links
HSPC300 network
kug network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0231153-1.PD.MW
Reference
Squarr et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
HSPC300 

dHSPC300

bait

MBP tag

kug 

dFAT2

prey

GST tag

kug 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
kug 
C-terminal region
sufficient binding region
aa 4307-4689

coordinates relative to kug-PC

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Components of the "WRC" complex were co-expressed in Sf9 cells (HSPC300, Sra-1, Abi, SCAR, Hem), pulled down via MBP tag, and further purified by size exclusion column. This entire WRC complex was used as bait for the pull down.

(Squarr et al., 2016)

Interaction in vitro; bait produced as a recombinant fusion protein in baculovirus-infected Sf9 insect cell system; prey produced as a recombinant fusion protein in bacterial system.

(Squarr et al., 2016)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)