FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
klhl10, soti
FlyBase ID
FBig0000104682
Interaction Network
Interactions Browser links
klhl10 network
soti network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0211287-1.Y2H
Reference
Kaplan et al., 2010
Description
physical association
Assay
Collection
Source/Stage
organism / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
klhl10 

Klhl10

bait

fused to GAL4 DNA-binding domain

klhl10 
klhl10 
soti 

Soti

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
klhl10 
interaction domain
sufficient binding region
aa 188-767
klhl10 
Kelch repeat domain
mutation disrupting interaction
aa 480aa 496aa 601

S480F

G496R

E601K

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from adult cDNA expression library).

(Kaplan et al., 2010)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Kaplan et al., 2010)
FBrf0211287-2.coIP.WB
Reference
Kaplan et al., 2010
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
soti 

Soti

bait

GFP tag

klhl10 

Klhl10

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Kaplan et al., 2010)
FBrf0211287-3.coIP.WB
Reference
Kaplan et al., 2010
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
klhl10 

Klhl10

bait

PtA tag

soti 

Soti

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Kaplan et al., 2010)
FBrf0231856-13.coIP.WB
Reference
Aram et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
soti 

Soti

bait

GFP tag

klhl10 

Klhl10

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Binding of klhl10 to soti is inhibited by cotransfection of increasing amounts of skap.

(Aram et al., 2016)

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Aram et al., 2016)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)