FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
egl, osk
FlyBase ID
FBig0000105078
Interaction Network
Interactions Browser links
egl network
osk network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0224776-9.coIP.PCR
Reference
Vazquez-Pianzola et al., 2014
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
egl 

Egl

bait
osk 

osk

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Vazquez-Pianzola et al., 2014)
FBrf0234579-38.coIP.SEQ
Reference
Vazquez-Pianzola et al., 2017
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
egl 

Egl

bait

GFP tag

osk 

osk

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Vazquez-Pianzola et al., 2017)
FBrf0234579-5.coIP.PCR
Reference
Vazquez-Pianzola et al., 2017
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
egl 

Egl

bait

GFP tag

osk 

osk

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Vazquez-Pianzola et al., 2017)
FBrf0248715-1.PD.A
Reference
Mohr et al., 2021
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
osk 

osk

bait

biotin label

osk 
osk 
osk 
osk 
osk 
egl 

EGL

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
osk 
3-prime UTR SL2a domain residues 533-652
sufficient binding region
nt 533-652

interaction at background level

osk 
3-prime UTR SL2a domain residues 550-597
mutation increasing interaction
nt 550-597
osk 
3-primeUTR SL2a domain residues 539-540
mutation decreasing interaction
nt 539-540

mutation of residues 539-540 returned interaction to background levels.

osk 
3-prime UTR TAS tl domain residues 533-545
sufficient binding region
nt 533-545

interaction is increased above background level.

osk 
3-prime UTR TAS tl domain residues 539-40
mutation decreasing interaction
nt 539-540

mutation of resides 539-540 within the context of the 533-545 TAS tl domain returns interaction to background level.

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced and labeled by in vitro transcription; prey produced and labeled by in vitro translation.

(Mohr et al., 2021)

SL2a interacts with egl at background level comparable to a TLS-deltaA/C mutant sequence common to multiple RNA stem-loops and was also used as a background level control.

(Mohr et al., 2021)
FBrf0250118-10a.coIP.PCR
Reference
Neiswender et al., 2021
Description
physical association
Assay
Collection
Source/Stage
ovary / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
egl 

Egl

bait

FLAG tag

egl 
osk 

osk

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
egl 
2pt interaction domain residues 965 and 969
mutation decreasing interaction
aa 965 and 969

S965K and S969R; coordinates relative to egl-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced by in vitro transcription; prey derived from transgenic adult female ovary extract.

(Neiswender et al., 2021)

Endogenous egl was depleted with shRNA. Introduction of a yeast leucine zipper to restore dimerization of egl 2pt mutant artificially, partially restores interaction with osk.

(Neiswender et al., 2021)
FBrf0256570-1.coIP.PCR
Reference
Gáspár et al., 2023
Description
physical association
Assay
Collection
Source/Stage
egg chamber / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
egl 

egl

bait

GFP tag

osk 

BB

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult female egg chambers; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Gáspár et al., 2023)

Interaction between egl and osk was increased with stau RNAi.

(Gáspár et al., 2023)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (5)