FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
His3, JIL-1
FlyBase ID
FBig0000106278
Interaction Network
Interactions Browser links
His3 network
JIL-1 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0207132-1.FW.WB
Reference
Bao et al., 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
His3 

H3

bait
JIL-1 

JIL-1

prey

GST tag

JIL-1 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
JIL-1 
C-terminal region
sufficient binding region
aa 927-1207

coordinates relative to JIL-1-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait derived from S2 nuclear extract; prey produced as a recombinant fusion protein in bacterial system.

(Bao et al., 2008)

Drosophila histones were fractionated by SDS-PAGE, blotted then incubated with GST-JIL-1. Bound GST-JIL-1 was detected by anti-GST mAb.

(Bao et al., 2008)
FBrf0207132-2.PD.WB
Reference
Bao et al., 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
His3 

H3

bait

GST tag

His3 
JIL-1 

JIL-1

prey

Myc tag

JIL-1 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
JIL-1 
C-terminal region
sufficient binding region
aa 927-1207

coordinates relative to JIL-1-PA

His3 
N-terminal tail
sufficient binding region
aa 1-74
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from transfected S2 cell extract.

(Bao et al., 2008)
FBrf0207132-3.PD.WB
Reference
Bao et al., 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
JIL-1 

JIL-1

bait

GST tag

JIL-1 
His3 

H3

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
JIL-1 
globular domain
sufficient binding region
aa 1144-1196

"G3" construct, coordinates relative to JIL-1-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from transfected S2 cell extract.

(Bao et al., 2008)
FBrf0218468-1.coIP.WB
Reference
Kellner et al., 2012
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Kc167
Participants
Corresponds to
Reported as
Role
Note
His3 

H3

bait
JIL-1 

Jil-1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of Kc167 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Kellner et al., 2012)

Antibody specific to phosphorylated His3 on amino acid S10 or S28 used for immunoprecipitation.

(Kellner et al., 2012)
FBrf0224797-1.ENZ.WB
Reference
Description
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
JIL-1 

JIL-1

enzyme

GST tag

JIL-1 

JIL-1

enzyme

GST tag

His3 

His3

enzyme target
His3 

His3

enzyme target
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
FBrf0224797-2.ENZ.A
Reference
Description
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
JIL-1 

JIL-1

enzyme

GST tag

JIL-1 

JIL-1

enzyme

GST tag

JIL-1 
His3 

His3

enzyme target

P[32] label

His3 

His3

enzyme target

P[32] label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
External Crossreferences and Linkouts ( 0 )
References (3)