FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Cpsf73, Sym
FlyBase ID
FBig0000108043
Interaction Network
Interactions Browser links
Cpsf73 network
Sym network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0208078-2.coIP.WB
Reference
Sullivan et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-Invitrogen
Participants
Corresponds to
Reported as
Role
Note
Cpsf73 

CPSF73

bait
Sym 

Symplekin

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Sullivan et al., 2009)
FBrf0208078-3.coIP.WB
Reference
Sullivan et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-Invitrogen
Participants
Corresponds to
Reported as
Role
Note
Sym 

Symplekin

bait
Cpsf73 

CPSF73

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Sullivan et al., 2009)
FBrf0229062-1.coIP.WB
Reference
Michalski and Steiniger, 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Sym 

Symp

bait

HA tag

Sym 
Cpsf73 

CPSF73

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Sym 
interaction domain
sufficient binding region
aa 272-1080

coordinates relative to Sym-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Sym, Cpsf73 and Cpsf100 were co-transfected.

(Michalski and Steiniger, 2015)

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Michalski and Steiniger, 2015)
FBrf0229062-6.coIP.WB
Reference
Michalski and Steiniger, 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Cpsf73 

CPSF73

bait

HA tag

Cpsf73 
Sym 

Symp

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Cpsf73 
C-terminal region
sufficient binding region
aa 466-684

coordinates relative to Cpsf73-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Sym, Cpsf73 and Cpsf100 were co-transfected.

(Michalski and Steiniger, 2015)

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Michalski and Steiniger, 2015)
FBrf0235253-10.coIP.WB
Reference
Harrington et al., 2017
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Sym 

Sym

bait

HA tag

Sym 
Cpsf73 

Cpsf73

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Sym 
interaction domain
sufficient binding region
aa 272-1165
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Harrington et al., 2017)
FBrf0235253-2.coIP.WB
Reference
Harrington et al., 2017
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Sym 

Sym

bait
Cpsf73 

Cpsf73

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Harrington et al., 2017)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)