FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
tral, me31B
FlyBase ID
FBig0000108569
Interaction Network
Interactions Browser links
tral network
me31B network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0190228-3.coIP.MS
Reference
Wilhelm et al., 2005
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
tral 

Tral

bait
me31B 

Me31B

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Wilhelm et al., 2005)
FBrf0190228-6.coIP.WB
Reference
Wilhelm et al., 2005
Description
physical association
Assay
Collection
Source/Stage
ovary / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
tral 

Tral

bait
me31B 

Me31B

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult female ovaries of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Wilhelm et al., 2005)

The tral-me31B interaction is RNA-independent (resistant to RNase treatment).

(Wilhelm et al., 2005)
FBrf0206982-10.coIP.WB
Reference
Tritschler et al., 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
tral 

Tral

bait

HA tag

tral 
me31B 

Me31B

prey

GFP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
tral 
FDF domain
sufficient binding region
aa 395-542

coordinates relative to tral-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Tritschler et al., 2008)
FBrf0206982-2.coIP.WB
Reference
Tritschler et al., 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
me31B 

Me31B

bait

HA tag

me31B 
tral 

Tral

prey

GFP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
me31B 
RecA domain
sufficient binding region
aa 268-459

coordinates relative to me31B-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene or transfected construct.

(Tritschler et al., 2008)
FBrf0210604-18.coIP.WB
Reference
Haas et al., 2010
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
me31B 

Me31B

bait

HA tag

tral 

Tral

prey

GFP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Haas et al., 2010)
FBrf0210604-19.coIP.WB
Reference
Haas et al., 2010
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
tral 

Tral

bait

HA tag

me31B 

Me31B

prey

GFP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Haas et al., 2010)
FBrf0215484-2.coIP.WB
Reference
Tritschler et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
me31B 

Me31B

bait

HA tag

me31B 
me31B 
tral 

Tral

prey

GFP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
me31B 
interaction domain residues 281,284
mutation disrupting interaction
aa 281,284

Q281A,H284A coordinates relative to me31B-PA

me31B 
interaction domain residues 288,292
mutation disrupting interaction
aa 288,292

T288A,K292A coordinates relative to me31B-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene or transfected construct.

(Tritschler et al., 2009)

Edc3 can compete with tral for binding to me31B.

(Tritschler et al., 2009)
FBrf0215484-4.coIP.WB
Reference
Tritschler et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
tral 

Tral

bait

HA tag

tral 
tral 
tral 
tral 
tral 
me31B 

Me31B

prey

GFP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
tral 
interaction domain residue 407
mutation disrupting interaction
aa 407

F407A, coordinates relative to tral-PA

tral 
interaction domain residue 408
mutation disrupting interaction
aa 408

D408A, coordinates relative to tral-PA

tral 
interaction domain residue 409
mutation disrupting interaction
aa 409

F409A, coordinates relative to tral-PA

tral 
interaction domain residue 406,408
mutation disrupting interaction
aa 406,408

D406K,D408K, coordinates relative to tral-PA

tral 
interaction domain residue 416
mutation disrupting interaction
aa 416

F416A, coordinates relative to tral-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Tritschler et al., 2009)
FBrf0236765-15.PD.WB
Reference
Götze et al., 2017
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
me31B 

Me31B

bait

GST tag

tral 

Tral

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of Sf21 cell line; bait produced as a recombinant fusion protein in baculovirus and Sf21 cell system; prey produced as a recombinant fusion protein in baculovirus and Sf21 cell system.

(Götze et al., 2017)
FBrf0236765-16.XL.MS
Reference
Götze et al., 2017
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
me31B 

Me31B

bait
me31B 
tral 

Tral

prey
tral 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
me31B 
cross-linked peptide
sufficient binding region
IEKELGTEIKPIPK
tral 
cross-linked peptide
sufficient binding region
SQLAKLK
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; proteins produced from endogenous genes.

(Götze et al., 2017)
FBrf0236770-28.coIP.WB
Reference
Wang et al., 2017
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
me31B 

ME31B

bait

GFP tag

tral 

TRAL

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Wang et al., 2017)
FBrf0244578-1.coIP.PCR
Reference
McCambridge et al., 2020
Description
physical association
Assay
Collection
Source/Stage
ovary / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
tral 

Tral

bait
me31B 

Me31B

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was wild-type adult ovaries; bait produced from endogenous gene; prey produced from endogenous gene.

(McCambridge et al., 2020)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (7)