FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
N, Traf6
FlyBase ID
FBig0000108767
Interaction Network
Interactions Browser links
N network
Traf6 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0226884-1.Y2H
Reference
Mishra et al., 2014
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
N 

Notch

bait

fused to LexA DNA-binding domain

N 
Traf6 

TRAF6

prey

fused to GAL4 activation domain

Traf6 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
N 
intracellular domain
sufficient binding region
aa 1765-1895

coordinates relative to N-PA

Traf6 
C-terminal region
sufficient binding region
aa 224-475

coordinates relative to Traf6-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast LexA-BD/GAL4-AD

(Mishra et al., 2014)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).

(Mishra et al., 2014)

8 Traf6 clones identified in this N two hybrid screen.

(Mishra et al., 2014)
FBrf0226884-3.PD.WB
Reference
Mishra et al., 2014
Description
physical association
Assay
Collection
Source/Stage
embryonic/larval salivary gland / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Traf6 

TRAF6

bait

GST tag

Traf6 
N 

Notch

prey
N 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
N 
intracellular domain
sufficient binding region
aa 1765-1895

coordinates relative to N-PA

Traf6 
C-terminal region
sufficient binding region
aa 202-475

coordinates relative to Traf6-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from transgenic larval salivary gland extract overexpressing N intracellular domain.

(Mishra et al., 2014)
FBrf0226884-4.coIP.WB
Reference
Mishra et al., 2014
Description
physical association
Assay
Collection
Source/Stage
embryonic/larval salivary gland / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Traf6 

TRAF6

bait

HA tag

Traf6 
N 

Notch

prey
N 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
N 
intracellular domain
sufficient binding region
aa 1765-1895

coordinates relative to N-PA

Traf6 
C-terminal region
sufficient binding region
aa 202-475

coordinates relative to Traf6-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was larval salivary glands of transgenic fly line; bait produced from tagged transgenic construct; prey produced from transgenic construct.

(Mishra et al., 2014)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)