FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
unc-13, Cam
FlyBase ID
FBig0000108862
Interaction Network
Interactions Browser links
unc-13 network
Cam network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0105342-12.FW.TV
Reference
Xu et al., 1998
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
unc-13 

dUNC13

bait

GST tag

unc-13 
Cam 

Calmodulin

prey

biotin label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
unc-13 
calmodulin-binding site
sufficient binding region
VLRQTEITARQRWHWAYNKIIMQLN
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey obtained from commercial source.

(Xu et al., 1998)
FBrf0105342-15.PD.WB
Reference
Xu et al., 1998
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Cam 

Calmodulin

bait

coupled to agarose beads

unc-13 

dUNC13

prey

GST tag

unc-13 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
unc-13 
calmodulin-binding site
sufficient binding region
VLRQTEITARQRWHWAYNKIIMQLN
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait obtained from commercial source; prey produced as a recombinant fusion protein in bacterial system.

(Xu et al., 1998)
FBrf0105342-8.LPD
Reference
Xu et al., 1998
Description
physical association
Assay
Collection
Source/Stage
retina / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Cam 

Calmodulin

bait

[125]I label

unc-13 

dUNC 13

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

2 unc-13 clones recovered in this phage display screen for Cam binding proteins.

(Xu et al., 1998)

Interaction in vitro; bait obtained from commercial source; prey produced as transgenic fusion protein in bacteriophage (prey from adult retina cDNA expression library).

(Xu et al., 1998)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)