FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000108913
Interaction Network
Interactions Browser links
Cam network
plx network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0105342-13.FW.TV
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
plx 

PLX

bait

GST tag

plx 
Cam 

Calmodulin

prey

biotin label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
plx 
calmodulin-binding site
sufficient binding region
RDAAELRELWRTAIRQTIMLNRME
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey obtained from commercial source.

FBrf0105342-16.PD.WB
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Cam 

Calmodulin

bait

coupled to agarose beads

plx 

PLX

prey

GST tag

plx 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
plx 
calmodulin-binding site
sufficient binding region
RDAAELRELWRTAIRQTIMLNRME
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait obtained from commercial source; prey produced as a recombinant fusion protein in bacterial system.

FBrf0105342-9.LPD
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Cam 

Calmodulin

bait

[125]I label

plx 

Pollux

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

1 plx clone recovered in this phage display screen for Cam binding proteins.

Interaction in vitro; bait obtained from commercial source; prey produced as transgenic fusion protein in bacteriophage (prey from adult retina cDNA expression library).

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)