FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000109379
Interaction Network
Interactions Browser links
endos network
tws network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0219186-1.PD.A
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

endos

bait

GST tag

tws 

tws

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
interaction domain residue 68
mutation increasing interaction
aa 68

S68D

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

FBrf0219186-3.PD.WB
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

endos

bait

GST tag

tws 

tws

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
interaction domain residue 68
mutation increasing interaction
aa 68

S68D

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

FBrf0251558-4.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
tws 

Tws

bait

GFP tag

Endos

prey

Flag tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
gwl phosphorylation site residue 68
mutation increasing interaction
aa 68

phosphomimetic mutation S68D; coordinates relative to endos-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was extract of an unspecified cell line; bait produced from transfected construct; prey produced from transfected construct.

FBrf0251558-5.PD.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

Endos

bait

GST tag

tws 

tws

prey

Flag tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
gwl phosphorylation site residue 68
mutation increasing interaction
aa 68

phosphomimetic mutation S68D; coordinates relative to endos-PA

central region deletion; residues 41-80
necessary binding region
aa 41-80

coordinates relative to endos-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from transfected construct.

FBrf0251558-6.ENZ.uv-vis
Reference
Description
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
tws 

Tws

enzyme

Flag tag

tws 

Tws

enzyme

Flag tag

Endos

enzyme regulator

GST tag

Endos

enzyme regulator

GST tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
FBrf0251558-7.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
tws 

Tws

bait

Endos

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was extract of an unspecified cell line; bait produced from transfected construct; prey produced from transfected construct.

constitutively active gwl in the cytoplasm increases interaction

FBrf0251558-8.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
tws 

Tws

bait

GFP tag

Endos

prey

Flag tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
gwl phosphorylation site residue 68
mutation increasing interaction
aa 68

phosphomimetic mutation S68D; coordinates relative to endos-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was extract of an unspecified cell line; bait produced from transfected construct; prey produced from transfected construct.

constitutively active gwl in the cytoplasm increases interaction when endo is expressed in the cytoplasm.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)