FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
IntS9, tut
FlyBase ID
FBig0000109566
Interaction Network
Interactions Browser links
IntS9 network
tut network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0234954-9.Y3H
Reference
Shan et al., 2017
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
IntS9 

IntS9

bait

fused to MS2 RNA stem loop

IntS9 
tut 

Tut

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
IntS9 
3-prime UTR
sufficient binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

RNA three-hybrid system: yeast LexA-MS2-BD/MS2-SL/GAL4-AD

(Shan et al., 2017)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Shan et al., 2017)

Binding of tut to 3\'UTR inhibits expression of luciferase reporter constructs.

(Shan et al., 2017)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)