Interaction in vitro; bait synthesized and labelled in vitro; prey derived from Kc cell nuclear extract.
Interaction abrogated by treatment with 0.1% NP40. Depletion of Slbp decreases interaction.
125nt from His3 pre-mRNA. 2\'O-methyl modification to prevent cleavage and biotin tag provided by oligo annealed to 3-prime end of His3 pre-mRNA.
Interaction in vitro; bait produced by in vitro transcription; prey derived from Kc nuclear extract.
63nt from His3 pre-mRNA with 2\'O-methyl modification to prevent cleavage
Interaction in vitro; bait commercially synthesized; prey derived from Kc nuclear extract.
