FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Madm, bun
FlyBase ID
FBig0000113758
Interaction Network
Interactions Browser links
Madm network
bun network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0210782-1.AF.MS
Reference
Gluderer et al., 2010
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
bun 

BunA

bait

HA or GFP tag

Madm 

Madm

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Gluderer et al., 2010)
FBrf0210782-2.coIP.WB
Reference
Gluderer et al., 2010
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Madm 

Madm

bait

HA tag

bun 

BunA

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Gluderer et al., 2010)
FBrf0210782-3.Y2H
Reference
Gluderer et al., 2010
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Madm 

Madm

bait

fused to GAL4 DNA-binding domain

Madm 
Madm 
bun 

BunA

prey

fused to GAL4 activation domain

bun 
bun 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
bun 
interaction domain
sufficient binding region
aa 475-553

coordinates relative to bun-PA

bun 
interaction domain residue 519
mutation decreasing interaction
aa 519

P519L, coordinates relative to bun-PA

Madm 
interaction domain
sufficient binding region
aa 458-566
Madm 
interaction domain residue 525
mutation disrupting interaction
aa 525

R525H

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Gluderer et al., 2010)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Gluderer et al., 2010)
External Crossreferences and Linkouts ( 0 )
References (1)