Source was larval brains of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from larval brain extract.
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
Two-hybrid system: yeast lexA-BD/B42-AD
Source was adult testis of transgenic fly line; proteins produced from tagged transgenic construct or endogenous gene.
The PLA signal develops only if probes to the two proteins are in close proximity to each other (within tens of nanometers).
Source was adult males testes; bait produced from transgenic construct; prey derived from endogenous gene.
