Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
Two-hybrid system: yeast GAL4-BD/GAL4-AD
coordinates relative to HP4-PA
coordinates relative to HP4-PA
coordinates relative to HP4-PA
coordinates relative to HP4-PA
coordinates relative to HP4-PA
coordinates relative to HP4-PA
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type salivary gland nuclear extract.
Source was larval salivary gland nuclei of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.
I191E, coordinates relative to Su(var)205-PA
W200A, coordinates relative to Su(var)205-PA
Bait fusion protein bound to lac0 sites and was visualized on polytene chromosomes. The ability of this chimera to recruit prey protein to lac0 sites was taken as evidence of direct physical interaction.
Source was larvae of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.
