FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Su(var)205, Hmt4-20
FlyBase ID
FBig0000116150
Interaction Network
Interactions Browser links
Su(var)205 network
Hmt4-20 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0208607-2.PBD.FM
Reference
Hines et al., 2009
Description
physical association
Assay
Collection
Source/Stage
salivary gland / larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Su(var)205 

HP1

bait

fused to lacI DNA binding domain

Su(var)205 
Su(var)205 
Su(var)205 
Su(var)205 
Hmt4-20 

Suv4-20

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Su(var)205 
CD domain
necessary binding region
aa 1-131

coordinates relative to Su(var)205-PA

Su(var)205 
CSD domain
necessary binding region
aa 82-206

coordinates relative to Su(var)205-PA

Su(var)205 
CSD domain residue 191
mutation disrupting interaction
aa 191

I191E, coordinates relative to Su(var)205-PA

Su(var)205 
CSD domain residue 200
mutation disrupting interaction
aa 200

W200A, coordinates relative to Su(var)205-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Bait fusion protein bound to lac0 sites and was visualized on polytene chromosomes. The ability of this chimera to recruit prey protein to lac0 sites was taken as evidence of direct physical interaction.

(Hines et al., 2009)

Source was larvae of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Hines et al., 2009)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)