FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
vig, His2A
FlyBase ID
FBig0000116436
Interaction Network
Interactions Browser links
vig network
His2A network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0238450-1.coIP.PCR
Reference
Tsui et al., 2018
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
vig 

Vig

bait

V5 tag

vig 
His2A 

H2A

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
vig 
C-terminal domain
necessary binding region
unspecified
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Tsui et al., 2018)
FBrf0238450-5.EMSA.A
Reference
Tsui et al., 2018
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
vig 

Vig

bait

His tag

His2A 

H2A

prey

[32]P label

His2A 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
His2A 
3-prime UTR
sufficient binding region
unspecified
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro transcription.

(Tsui et al., 2018)
External Crossreferences and Linkouts ( 0 )
References (1)