FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
ari-1, koi
FlyBase ID
FBig0000116512
Interaction Network
Interactions Browser links
ari-1 network
koi network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0238699-1.coIP.WB
Reference
Tan et al., 2018
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
koi 

Koi

bait

HA or GFP tag

koi 
koi 
ari-1 

Ari-1

prey

V5 tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
koi 
C-terminal domain
sufficient binding region
aa 563-967

coordinates relative to koi-PA

koi 
N-terminal domain
sufficient binding region
aa 1-441

coordinates relative to koi-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Tan et al., 2018)
FBrf0238699-2.coIP.WB
Reference
Tan et al., 2018
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
ari-1 

Ari-1

bait

V5 tag

koi 

Koi

prey

HA tag

koi 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
koi 
C-terminal domain
sufficient binding region
aa 563-967

coordinates relative to koi-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Tan et al., 2018)
FBrf0238699-3.ENZ.WB
Reference
Description
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
ari-1 

Ari-1

enzyme

GST tag

ari-1 

Ari-1

enzyme

GST tag

ari-1 
ari-1 
ari-1 
koi 

Koi

enzyme target

T7 tag

koi 

Koi

enzyme target

T7 tag

koi 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)