FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
ari-1, park
FlyBase ID
FBig0000116544
Interaction Network
Interactions Browser links
ari-1 network
park network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0238699-4.coIP.WB
Reference
Tan et al., 2018
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
park 

Park

bait

FLAG or HA tag

ari-1 

Ari-1

prey

V5 tag

ari-1 
ari-1 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ari-1 
Ring1 domain residue 136
mutation increasing interaction
aa 136

C136Y

ari-1 
IBR and Ring2 domains residues 187,332
mutation increasing interaction
aa 187,332

V187E,S332P

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Tan et al., 2018)
FBrf0238699-5.coIP.WB
Reference
Tan et al., 2018
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
ari-1 

Ari-1

bait

V5 tag

ari-1 
ari-1 
park 

Park

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ari-1 
Ring1 domain residue 136
mutation increasing interaction
aa 136

C136Y

ari-1 
IBR and Ring2 domains residues 187,332
mutation increasing interaction
aa 187,332

V187E,S332P

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Tan et al., 2018)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)