FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000116595
Interaction Network
Interactions Browser links
Cam network
eag network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0174770-3.PD.WB
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
eag 

eag

bait

GST tag

eag 
eag 
Cam 

CaM

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
eag 
interaction domain
sufficient binding region
aa 556-803

coordinates relative to eag-PA

eag 
interaction domain residues 732,735
mutation disrupting interaction
aa 732,735

F732S,F735S, coordinates relative to eag-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in baculovirus and Sf21 cell system.

FBrf0238832-1.PD.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Cam 

CaM

bait
eag 

eag

prey
eag 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
eag 
CaM binding domain residues 731,734
mutation disrupting interaction
aa 731,734

F731S,F734S, coordinates relative to eag-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait source unspecified; prey derived from wild-type and mutant adult head extract.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)