FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
N, Vps35
FlyBase ID
FBig0000116934
Interaction Network
Interactions Browser links
N network
Vps35 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0240088-2.coIP.WB
Reference
Li et al., 2018
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Vps35 

Vps35

bait

FLAG tag

N 

NICD

prey

V5 tag

N 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
N 
intracellular domain
sufficient binding region
aa 1771-2703
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of HEK293T cell line; bait produced from transfected construct; prey produced from transfected construct.

(Li et al., 2018)
FBrf0240088-4.coIP.WB
Reference
Li et al., 2018
Description
physical association
Assay
Collection
Source/Stage
brain / larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
N 

NICD

bait

V5 tag

Vps35 

Vps35

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was larval brains of transgenic fly line; bait produced from tagged transgenic construct; prey produced from tagged transgenic construct.

(Li et al., 2018)
External Crossreferences and Linkouts ( 0 )
References (1)