FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000117425
Interaction Network
Interactions Browser links
eag network
CaMKII network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0174770-1.coIP.WB
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
eag 

eag

bait

CaMKII

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
interaction domain
mutation increasing interaction
aa 287

T287D, mutation increases binding in absence of Ca2+, coordinates relative to CaMKII-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult heads of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

The presence of Ca2+ enhances the eag-CaMKII interaction.

FBrf0174770-2.PD.WB
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
eag 

eag

bait

GST tag

eag 
eag 
eag 

CaMKII

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
eag 
interaction domain
sufficient binding region
aa 761-803

coordinates relative to eag-PA

eag 
interaction domain
necessary binding region
aa 773-794

coordinates relative to eag-PA

interaction domain
mutation increasing interaction
aa 287

T287D, mutation allows binding in absence of Ca2+ and in presence of T306D,T307D mutation, coordinates relative to CaMKII-PA

CaM binding domain
mutation disrupting interaction
aa 306,307

T306D,T307D, coordinates relative to CaMKII-PA

catalytic domain
mutation disrupting interaction
aa 206

I206K, coordinates relative to CaMKII-PA

eag 
interaction domain
mutation decreasing interaction
aa 784,785aa 789,790aa 782,783aa 794,795

R784K/Q785K, coordinates relative to eag-PA

D789K/E790K, coordinates relative to eag-PA

L782K/A783K, coordinates relative to eag-PA

G794K/E795K, coordinates relative to eag-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in baculovirus and Sf21 cell system.

FBrf0174770-4.ENZ.A
Reference
Description
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

CaMKII

enzyme

CaMKII

enzyme
eag 

eag

enzyme target

GST tag, P[32] label

eag 

eag

enzyme target

GST tag, P[32] label

eag 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
FBrf0240941-1.ITC
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

CaMKII

bait
eag 

dEAG

prey

MBP tag

eag 
eag 
eag 
eag 
eag 
eag 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
kinase domain
sufficient binding region
aa 1-283
eag 
interaction domain
sufficient binding region
aa 768-795
eag 
interaction domain residue 782
mutation decreasing interaction
aa 782

L782A

eag 
interaction domain residue 784
mutation decreasing interaction
aa 784

R784A

eag 
interaction domain residue 788
mutation decreasing interaction
aa 788

I788A

eag 
interaction domain residue 785
mutation decreasing interaction
aa 785

Q785A

eag 
interaction domain residue 789
mutation decreasing interaction
aa 789

D789A

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

FBrf0240941-2.XC
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

CaMKII

neutral component

CaMKII

neutral component

CaMKII

neutral component
eag 

dEAG

neutral component
eag 
eag 

dEAG

neutral component
eag 

dEAG

neutral component
eag 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
kinase domain
sufficient binding region
aa 1-283
eag 
interaction domain
sufficient binding region
aa 768-820
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; proteins produced as recombinant fusion proteins in bacterial system.

External Crossreferences and Linkouts ( 0 )
References (2)