FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Shark, Dok
FlyBase ID
FBig0000117584
Interaction Network
Interactions Browser links
Shark network
Dok network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0190273-1.Y2H
Reference
Biswas et al., 2006
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Shark 

Shark

bait

fused to LexA DNA-binding domain

Shark 
Dok 

Ddok

prey

fused to B42 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Shark 
N-terminal domain
sufficient binding region
aa 10-468
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast LexA-BD/B42-AD. An activated Src kinase domain was coexpressed with two-hybrid components to increase phosphorylation of library proteins.

(Biswas et al., 2006)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).

(Biswas et al., 2006)
FBrf0190273-2.Y2H
Reference
Biswas et al., 2006
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Shark 

Shark

bait

fused to LexA DNA-binding domain

Shark 
Dok 

Ddok

prey

fused to B42 activation domain

Dok 
Dok 
Dok 
Dok 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Shark 
N-terminal domain
sufficient binding region
aa 10-468
Dok 
C-terminal domain residue 499
mutation disrupting interaction
aa 499

Y499F

Dok 
C-terminal domain residue 427
mutation decreasing interaction
aa 427

Y427F

Dok 
C-terminal domain residue 515
mutation decreasing interaction
aa 515

Y515F

Dok 
C-terminal domain residue 537
mutation decreasing interaction
aa 537

Y537F

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast LexA-BD/B42-AD. An activated Src kinase domain was coexpressed with two-hybrid components to increase phosphorylation of library proteins.

(Biswas et al., 2006)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein.

(Biswas et al., 2006)
FBrf0190273-3.coIP.WB
Reference
Biswas et al., 2006
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Dok 

Ddok

bait

FLAG tag

Dok 
Shark 

Shark

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Dok 
C-terminal domain residue 499,515
mutation disrupting interaction
aa 499,515

Y499F,Y515F

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from endogenous gene or transfected construct; prey produced from endogenous gene or transfected construct.

(Biswas et al., 2006)
External Crossreferences and Linkouts ( 0 )
References (1)