FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
ImpL2, Ilp2
FlyBase ID
FBig0000117677
Interaction Network
Interactions Browser links
ImpL2 network
Ilp2 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0204716-1.PD.A
Reference
Honegger et al., 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Ilp2 

Dilp

bait

FLAG tag

ImpL2 

Imp-L2

prey

[35]S label

ImpL2 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ImpL2 
interaction domain
mutation disrupting interaction
aa 232

stop codon introduced at aa 232, coordinates relative to ImpL2-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Honegger et al., 2008)
FBrf0212768-1.FW
Reference
Alic et al., 2011
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
ImpL2 

ImpL2

bait

Myc tag

Ilp2 

Ilp2

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait secreted into S2 cell culture medium; prey derived from wild-type adult head extract.

(Alic et al., 2011)

Adult head proteins were separated by PAGE, transferred to nitrocellulose and probed with Myc-tagged ImpL2. Ilp2 identified by disappearance of band in Ilp2 mutant extracts.

(Alic et al., 2011)
FBrf0218395-3209.DPiM
Reference
Guruharsha et al., 2011
Description
physical association
Assay
Collection
DPiM-2
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
ImpL2 
unspecified role
ImpL2 
unspecified role
ImpL2 
unspecified role
Ilp2 
unspecified role
Ilp2 
unspecified role
Ilp2 
unspecified role
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

HGScore = 317.04122

FBrf0220509-8.coIP.WB
Reference
Okamoto et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Ilp2 

Dilp2

bait

His tag

ImpL2 

Imp-L2

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

ImpL2 binds most Ilp proteins tested, except for Ilp7.

(Okamoto et al., 2013)

The Sdr and ImpL2 bind Ilp family proteins with different binding affinities.

(Okamoto et al., 2013)

Sdr and ImpL2 do not bind each other.

(Okamoto et al., 2013)

ImpL2 and Sdr compete for binding to Ilp2.

(Okamoto et al., 2013)

Source was cell extract of HEK293T cell line; bait produced from transfected construct; prey produced from transfected construct.

(Okamoto et al., 2013)
FBrf0240146-5.SPR
Reference
Roed et al., 2018
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
ImpL2 

Imp-L2

bait
Ilp2 

DILP2

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in baculovirus and Sf9 system; prey produced as a yeast expression system.

(Roed et al., 2018)
External Crossreferences and Linkouts ( 0 )
References (5)