FB2024_03 , released June 25, 2024
Physical Interaction report
Open Close
General Information
Interaction Type
physical association
Interacting Genes
N, htk
FlyBase ID
FBig0000119852
Interaction Network
Interactions Browser links
N network
htk network
esyN Network Diagram
Show neighbor-neighbor interactions:
Select Layout:
Legend:
Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0243018-1.Y2H
Reference
Singh et al., 2019
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
N 

N

bait

fused to LexA DNA-binding domain

N 
htk 

htk

prey

fused to GAL4 activation domain

htk 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
N 
intracellular domain
sufficient binding region
aa 1765-1895
htk 
C-terminal domain
sufficient binding region
aa 2424-2486
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast LexA-BD/GAL4-AD

(Singh et al., 2019)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).

(Singh et al., 2019)
FBrf0243018-2.coIP.WB
Reference
Singh et al., 2019
Description
physical association
Assay
Collection
Source/Stage
wing disc / larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
htk 

Htk

bait

HA tag

N 

NICD

prey
N 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
N 
intracellular domain
sufficient binding region
unspecified
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was larval wing discs of transgenic fly line; bait produced from tagged transgenic construct; prey produced from transgenic construct or endogenous gene.

(Singh et al., 2019)
FBrf0243018-3.coIP.WB
Reference
Singh et al., 2019
Description
physical association
Assay
Collection
Source/Stage
wing disc / larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
N 

NICD

bait
N 
htk 

Htk

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
N 
intracellular domain
sufficient binding region
unspecified
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was larval wing discs of transgenic fly line; bait produced from transgenic construct; prey produced from tagged transgenic construct.

(Singh et al., 2019)
FBrf0243018-4.coIP.WB
Reference
Singh et al., 2019
Description
physical association
Assay
Collection
Source/Stage
salivary gland / larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
htk 

Htk

bait
N 

NICD

prey
N 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
N 
intracellular domain
sufficient binding region
unspecified
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was larval salivary gland of transgenic fly line; bait produced from endogenous gene; prey produced from transgenic construct.

(Singh et al., 2019)
External Crossreferences and Linkouts ( 0 )
References (1)