Source was embryonic nuclear extract of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.
Source was embryonic nuclear extract of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.
Interaction in vitro; proteins co-produced and purified as recombinant fusion proteins in a baculovirus and Sf21 cell system.
Interaction in vitro; proteins co-produced and purified as recombinant fusion proteins in a baculovirus and Sf21 cell system.
Source was ovaries of genetically engineered fly line; bait produced from gene tagged at the endogenous locus; prey produced from endogenous gene.
Two-hybrid system: yeast lexA-BD/GAL4-AD
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
coordinates relative to JIL-1-PA, Kd = 0.8uM
coordinates relative to JIL-1-PA, Kd = 120uM
F989A, coordinates relative to JIL-1-PA, Kd = 122uM
G991V, coordinates relative to JIL-1-PA
F992A, coordinates relative to JIL-1-PA
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.
