FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
MED19, pnr
FlyBase ID
FBig0000120617
Interaction Network
Interactions Browser links
MED19 network
pnr network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0246810-1.coIP.WB
Reference
Immarigeon et al., 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
MED19 

Med19

bait
pnr 

Pnr

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from transfected construct.

(Immarigeon et al., 2020)
FBrf0246810-2.coIP.WB
Reference
Immarigeon et al., 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
pnr 

Pnr

bait

Myc tag

MED19 

Med19

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Immarigeon et al., 2020)
FBrf0246810-3.PD.A
Reference
Immarigeon et al., 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
pnr 

Pnr

bait

GST tag

MED19 

Med19

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Immarigeon et al., 2020)
FBrf0246810-4.PD.A
Reference
Immarigeon et al., 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
MED19 

Med19

bait

GST tag

pnr 

Pnr

prey

[35]S label

pnr 
pnr 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
pnr 
interaction domain
sufficient binding region
aa 130-278
pnr 
C-ZF domain
necessary binding region
aa 220-269
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Immarigeon et al., 2020)
FBrf0246810-5.BiFC.FM
Reference
Immarigeon et al., 2020
Description
physical association
Assay
Collection
Source/Stage
wing disc / larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
pnr 

Pnr

bait

YFP tag, N-terminal fragment

MED19 

Med19

prey

YFP tag, C-terminal fragment

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was wing discs of transgenic fly line; bait produced from tagged transgenic construct; prey produced from tagged transgenic construct.

(Immarigeon et al., 2020)
FBrf0246810-6.PD.WB
Reference
Immarigeon et al., 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
MED19 

Med19

bait

GST tag

MED19 
pnr 

Pnr

prey

HA tag

pnr 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
pnr 
N-terminal domain
sufficient binding region
aa 1-291
MED19 
interaction domain
necessary binding region
aa 126-165,206-220
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced by in vitro translation.

(Immarigeon et al., 2020)
External Crossreferences and Linkouts ( 0 )
References (1)