FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
vap, btl
FlyBase ID
FBig0000120906
Interaction Network
Interactions Browser links
vap network
btl network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0179968-2.PD.WB
Reference
Woodcock and Hughes, 2004
Description
physical association
Assay
Collection
Source/Stage
organism / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
vap 

p120 RasGap

bait

GST tag

vap 
btl 

btl

prey

Myc tag

btl 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
vap 
SH232 domain
mutation disrupting interaction
aa 110,278

R110L, R278L, coordinates relative to vap-PA

btl 
intracellular region
sufficient binding region
unspecified
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

btl transgenic construct is fused to the transmembrane domain of an over-expression form of Tor ( btl::tor , FBgn0014948)

(Woodcock and Hughes, 2004)

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from transgenic construct

(Woodcock and Hughes, 2004)
FBrf0179968-3.PD.WB
Reference
Woodcock and Hughes, 2004
Description
physical association
Assay
Collection
Source/Stage
organism / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
btl 

btl

bait

biotinylation

btl 
vap 

p120 RasGap

prey

Myc tag

vap 
vap 
vap 
vap 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
btl 
conserved juxtamembrane sequence
sufficient binding region
aa 694-706

coordinates relative to btl-PA

vap 
SH232 domain
sufficient binding region
aa 83-343

coordinates relative to vap-PA

vap 
SH232 domain residue 110
mutation disrupting interaction
aa 110

R110L, coordinates relative to vap-PA

vap 
SH232 domain
mutation disrupting interaction
aa 110,278

R110L, R278L, coordinates relative to vap-PA

vap 
SH232 domain residue 278
mutation decreasing interaction
aa 278

R278L, coordinates relative to vap-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait was synthesized peptides; prey derived from transgenic construct

(Woodcock and Hughes, 2004)
External Crossreferences and Linkouts ( 0 )
References (1)