FB2024_03 , released June 25, 2024
Physical Interaction report
Open Close
General Information
Interaction Type
physical association
Interacting Genes
wg, N
FlyBase ID
FBig0000121586
Interaction Network
Interactions Browser links
wg network
N network
esyN Network Diagram
Show neighbor-neighbor interactions:
Select Layout:
Legend:
Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0111069-3.coIP.WB
Reference
Wesley, 1999
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
wg 

Wg

bait
N 

N

prey
N 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
N 
EGF repeat domain
necessary binding region
aa 19-36aa 1-18

coordinates relative to N-PA

coordinates relative to N-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Wesley, 1999)

Interaction requires calcium; Deletion of N EGF repeat domain aa 1-18 results in loss of one of the two wg - N complexes and deletion of aa 19-36 results in loss of both complexes.

(Wesley, 1999)
FBrf0111069-4.coIP.WB
Reference
Wesley, 1999
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
wg 

Wg

bait
N 

N

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Full-length N interacts with wg in 6-12 hr embryos.

(Wesley, 1999)

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Wesley, 1999)
FBrf0111069-5.coIP.WB
Reference
Wesley, 1999
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
wg 

Wg

bait
N 

N

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Wesley, 1999)

Truncated N interacts with wg in 0-3 hr embryos.

(Wesley, 1999)
External Crossreferences and Linkouts ( 0 )
References (1)