Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild type testes extract.
Interaction in vitro; bait and prey produced as recombinant fusion proteins in bacterial system.
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
Two-hybrid system: yeast LexA-BD/GAL4-AD