FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Smurf, IntS9
FlyBase ID
FBig0000121973
Interaction Network
Interactions Browser links
Smurf network
IntS9 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0255681-17.coIP.WB
Reference
Liu et al., 2023
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
IntS9 

IntS9

bait

FLAG tag

Smurf 

Smurf

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

An enzymatically inactive Smurf construct was used.

(Liu et al., 2023)

Source was cell extract of S2 cell line; bait and prey produced from transfected constructs.

(Liu et al., 2023)
FBrf0255681-20.coIP.WB
Reference
Liu et al., 2023
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Smurf 

Smurf

bait
IntS9 

IntS9

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait and prey produced from endogenous genes.

(Liu et al., 2023)
FBrf0255681-31.coIP.WB
Reference
Liu et al., 2023
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Smurf 

Smurf

bait

FLAG tag

IntS9 

IntS9

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait and prey produced from transfected constructs.

(Liu et al., 2023)
External Crossreferences and Linkouts ( 0 )
References (1)