FB2024_03 , released June 25, 2024
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
IntS9, IntS4
FlyBase ID
FBig0000122038
Interaction Network
Interactions Browser links
IntS9 network
IntS4 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0250625-1.Y2H
Reference
Albrecht et al., 2018
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
IntS9 

dIntS9

bait

fused to GAL4 DNA-binding domain

IntS4 

dIntS4

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Albrecht et al., 2018)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Albrecht et al., 2018)
FBrf0254630-1.PD.MW
Reference
Lin et al., 2022
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
IntS4 

IntS4

neutral component

His tag

IntS4 

IntS4

neutral component

His tag

IntS4 

IntS4

neutral component

His tag

IntS9 

IntS9

neutral component
IntS9 

IntS9

neutral component
IntS9 

IntS9

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

IntS4, IntS9 and IntS11 were co-expressed and co-purified.

(Lin et al., 2022)

Interaction in vitro; proteins produced as a recombinant fusion proteins in baculovirus cell system.

(Lin et al., 2022)
FBrf0254630-10.PD.WB
Reference
Lin et al., 2022
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
IntS9 

IntS9

neutral component

FLAG tag

IntS9 

IntS9

neutral component

FLAG tag

IntS9 

IntS9

neutral component

FLAG tag

IntS4 

Int4

neutral component
IntS4 

Int4

neutral component
IntS4 

Int4

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of DL1 cell line; bait produced from transfected construct. Prey produced from endogenous gene.

(Lin et al., 2022)
FBrf0254630-3.PD.EM
Reference
Lin et al., 2022
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
IntS4 

IntS4

neutral component

His tag

IntS4 

IntS4

neutral component

His tag

IntS4 

IntS4

neutral component

His tag

IntS9 

IntS9

neutral component
IntS9 

IntS9

neutral component
IntS9 

IntS9

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; proteins produced as a recombinant fusion proteins in baculovirus cell system.

(Lin et al., 2022)

IntS4, IntS9 and IntS11 were co-expressed and co-purified.

(Lin et al., 2022)
External Crossreferences and Linkouts ( 0 )
References (2)