FB2024_03 , released June 25, 2024
Reagent: BS_cDNA
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General Information
Name
BS_cDNA
Species
D. melanogaster
Reagent type
cDNA library
FlyBase ID
FBlc0000002
Project
Created by
Justen Andrews in Brian Oliver's laboratory at the National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK), National Institutes of Health (NIH), Bethesda, MD, USA.
Vector
Title
BS cDNA library of D. melanogaster, adult male, testis including ejaculatory duct and seminal vesicle.
Accessions
Overview
Description

Cloned cDNAs prepared from polyadenylated RNA isolated from young adult male testes, seminal vesicles, and ejaculatory ducts.

(Andrews et al., 2000)
Available from
Biosample Source
Overview
Strain
Stage
adult stage
(Andrews et al., 2000)
Sex
male
(Andrews et al., 2000)
Tissue isolated
ejaculatory duct
(Andrews et al., 2000)
testis
(Andrews et al., 2000)
seminal vesicle
(Andrews et al., 2000)
Other tissues studied
Cell component
Cell line
Key genes
Methods
Sample preparation

Adult male testes were dissected from 1-5 day old y[1] w[67c1] Drosophila melanogaster flies, after removal of the paragonia and inclusion of the ejaculatory ducts and seminal vesicles.

Biosamples used (1)
Biosample
Type
Title
BS_BS_cDNA
tissue
D. melanogaster, adult male, testis including ejaculatory duct and seminal vesicle, source for cDNA.
Reagent Details
Methods
poly(A) RNA isolation
(Andrews et al., 2000)
size fractionation
(Andrews et al., 2000)
Molecular Construct used
Protocol

Poly(A)+ RNA was purified from dissected testis. The size fractionated cDNA (1-6kb) was directionally cloned in the Stratagene Uni-Zap XR vector using EcoRI and XhoI restriction sites. pBluescript SK (-) is produced after excision. The host strain is E.coli SOLR.

(Andrews et al., 2000)
Mode of Assay
Data analysis
Comments
Associated Data
Size
Associated features
6,597 Clone(s)
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Files
Additional Information

[BDGP: other ESTs](http://www.fruitfly.org/EST/otherEST.html)

Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
BS
BS_cDNA
Name Synonyms
BS cDNA library of D. melanogaster, adult male, testis including ejaculatory duct and seminal vesicle.
(Andrews et al., 2000)
Secondary FlyBase IDs
    References (1)