FB2024_03 , released June 25, 2024
Reagent: MIP_cDNA
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General Information
Name
MIP_cDNA
Species
D. melanogaster
Reagent type
cDNA library
FlyBase ID
FBlc0000052
Project
Created by
[BDGP: cDNA & EST FAQ](http://www.fruitfly.org/EST/faq.html)
Vector
Title
cDNA library of D. melanogaster, isolated by an inverse-PCR screen (SLIP) from other cDNA libraries.
Accessions
    Overview
    Description

    Clones isolated by an inverse-PCR screen (SLIP) using a mixture of the GH, LD, LP, and SD cDNA libraries.

    Available from
    Biosample Source
    Overview
    Strain
    iso-1
    (Rubin et al., 2000)
    Stage
    larval stage
    (Rubin et al., 2000)
    pupal stage
    (Rubin et al., 2000)
    adult stage
    (Rubin et al., 2000)
    Sex
    Tissue isolated
    adult head
    (Rubin et al., 2000)
    organism
    (Rubin et al., 2000)
    Other tissues studied
    Cell component
    Cell line
    Schneider's-line-2
    (Rubin et al., 2000)
    Key genes
    Methods
    Sample preparation

    Adult heads were collected from an isogenic y; cn bw sp (iso-1) strain.

    Biosamples used (4)
    Biosample
    Type
    Title
    BS_GH_cDNA
    tissue
    D. melanogaster, iso-1 strain, adult head, source for cDNA.
    BS_LD_cDNA
    whole organism
    D. melanogaster, iso-1 strain, embryo (0-22 hr), source for cDNA.
    BS_LP_cDNA
    whole organism
    D. melanogaster, iso-1 strain, larva and pupa, source for cDNA.
    BS_SD_cDNA
    immortalized cell line
    D. melanogaster, Schneider's Line-2 cells, source for cDNA.
    Reagent Details
    Methods
    Molecular Construct used
    Protocol

    These clones have been isolated using a directed screening process called SLIP (Self-Ligation of Inverse PCR Products). Clones isolated from this screen have been PCR-amplified using a mixture of 4 different plasmid cDNA libraries as template. These include the GH, LD, LP, and SD cDNA libraries.

    (FlyBase Curators, 2016-)
    Mode of Assay
    Data analysis
    Comments
    Associated Data
    Size
    Associated features
    1,992 Clone(s)
    Download 
    Files
    Additional Information

    [BDGP: Inverse-PCR Screen, SLIP](http://www.fruitfly.org/EST/SLIPscreen.html)

    Synonyms and Secondary IDs (3)
    Reported As
    Symbol Synonym
    MIP
    MIP_cDNA
    Name Synonyms
    cDNA library of D. melanogaster, isolated by an inverse-PCR screen (SLIP) from other cDNA libraries.
    (FlyBase Curators, 2016-)
    Secondary FlyBase IDs
      References (1)