A set of transgenic insertion stocks derived by TE mobilization using the Dhyd\Minos-based construct Mi{ET1}; vetted by the Gene Disruption Project (GDP). The Mi{ET1} construct carries the Avic\GFPE.3xP3 fluorescent marker, a Scer\GAL4 driver/reporter gene, and bacterial sequences that allow plasmid rescue.
Additional Mi{ET1} insertion lines were generated, mapped, and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank.
A genetic screen allowing recovery of insertions on all chromosomes was carried out in a w1118 isogenic background (FBrf0179424). Mobilization of the Minos element occurred in animals carrying Mi{ET1} on TM3-SbSer, a third chromosome balancer chromosome and P{hsILMiT}2.4, a stable source of Minos transposase, on an second chromosome balancer chromosome, CyO-MiT2.4.
Flanking genomic sequences were obtained by sequencing of inverse PCR products, and were mapped by alignment to the genomic sequence. 10,781 insertions from 10,630 strains were mapped to a unique site in the genome. Lines selected for the Gene Disruption Project collection were balanced and their insertions sites verified by resequencing. Stocks were generated and balanced in the w1118 isogenic background.
