A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{EPg}. The P{EPg} construct carries the mini-white marker w+mC, and Scer\UAS binding sites for the Scer\GAL4 transcriptional regulator (FBrf0128569). The GAL4-UAS system allows regulated expression of genes proximate to the site of the insertion: genes properly oriented with respect to the Scer\UAS sequences can be conditionally expressed via transgene-derived Scer\GAL4 activity.
Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.
More than 8,500 independent insertions were generated.
Markers in stocks (as originally submitted to the BDSC):w1118.
Flanking genomic sequences were determined by sequencing of inverse PCR products, and were mapped by alignment to the genomic sequence.
Lines donated by DeveloGen (now Evotec) were added to the Gene Disruption Project collection.
P{EPg} is similar to P{EP}, but has been modified to allow activation of the Scer\GAL4 driver in the female germline (FBrf0128569).
