FB2024_02 , released April 23, 2024
Reagent: CHORI-322_BAC
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General Information
Name
CHORI-322_BAC
Species
D. melanogaster
Reagent type
genomic clone collection
FlyBase ID
FBlc0000784
Project
Created by
Vector
    Title
    Pacman genomic clone collection for D. melanogaster, iso-1 strain, adult, 21kb inserts.
    Accessions
    Overview
    Description

    A BAC library containing genomic DNA fragments (average size of 21 kb) in the attB-P[acman]-CmR-BW vector for efficient transgenesis.

    (Venken et al., 2009)
    Available from
    [Pacman Libraries](http://flypush.imgen.bcm.tmc.edu/pacmanfly/libraries.html)
    (Venken et al., 2009)
    Biosample Source
    Overview
    Strain
    iso-1
    (Venken et al., 2009)
    Stage
    adult stage
    (Venken et al., 2009)
    Sex
    Tissue isolated
    organism
    (Venken et al., 2009)
    Other tissues studied
    Cell component
    Cell line
    Key genes
    Methods
    Sample preparation
    Biosamples used (1)
    Biosample
    Type
    Title
    BS_CHORI-322_BAC
    whole organism
    D. melanogaster, iso-1 strain, adult, source for BAC genomic clones.
    Reagent Details
    Methods
    size fractionation
    (Venken et al., 2009)
    Protocol

    DNA isolated from adult fly tissue was prepared in agarose blocks and partially digested with MboI. 20-25 kb fragments were size-selected by contour-clamped homogeneous electric field (CHEF) gel electrophoresis, and ligated into the BamHi site of the attB-P[acman]-CmR-BW vector, a special adaptation of the P[acman] vector. Libraries were transformed into the E.coli strain "EPI300-T1R".

    (Venken et al., 2009)
    Mode of Assay

    The ends of the BAC inserts were sequenced (paired ends) and aligned to the reference genome assembly.

    (Venken et al., 2009)
    Data analysis
    Comments

    A total of 73,728 clones were generated.

    (Venken et al., 2009)
    Associated Data
    Size
    Associated features
    36,966 Clone(s)
    Download 
    Files
    Additional Information
    Synonyms and Secondary IDs (3)
    Reported As
    Symbol Synonym
    CHORI-322
    (Venken et al., 2009)
    CHORI-322_BAC
    Name Synonyms
    Pacman genomic clone collection for D. melanogaster, iso-1 strain, adult, 21kb inserts.
    (Venken et al., 2009)
    Secondary FlyBase IDs
      References (3)