FB2024_02 , released April 23, 2024
Reagent: TRiP-OE-VPR
Open Close
General Information
Name
TRiP-OE-VPR
Species
D. melanogaster
Reagent type
construct collection
FlyBase ID
FBlc0003381
Project
Created by
DRSC
Vector
    Title
    A set of transgenic sgRNA constructs for targeted gene overexpression by dCas9-VPR.
    Accessions
      Overview
      Description

      A set of transgenic constructs that each consist of two single guide RNAs (sgRNAs) targeting about 500bp upstream of the transcription start site of the targeted gene. The two sgRNAs are under the control of two independent U6 (ubiquitous) promoters. When crossed to a stock that contains a source of nuclease-dead Cas9 fused to a transcriptional activation domain, such as dCas9-VPR, the targeted gene will be overexpressed wherever the dCas9-activator is expressed.

      (Transgenic RNAi Project members, 2017-)
      Available from
      Bloomington Drosophila Stock Center
      Biosample Source
      Overview
      Strain
      Stage
      Sex
      Tissue isolated
      Other tissues studied
      Cell component
      Cell line
      Key genes
      Methods
      Sample preparation
      Reagent Details
      Methods
      Protocol

      pCFD4 vector described at [CRISPR fly design](http://www.crisprflydesign.org/plasmids/)

      (Transgenic RNAi Project members, 2017-)
      Mode of Assay
      Data analysis
      Comments

      Many of the TRiP RNAi and sgRNA stocks generated at Harvard Medical School (HMS lines) and Tsinghua University (HMC lines), but not stocks generated at the National Institute of Genetics in Japan (HMJ lines), contain the sev21 allele as a result of introducing a mutated X chromosome from Bloomington stock 35781 (FBst0035781) or 32261 (FBst0032261) as the transgenes were being established in stock.

      (Escobedo et al., 2019)
      Associated Data
      Size
      Associated features
      1,547 Transgenic Construct(s)
      Download 
      3,094 sgRNA(s)
      Download 
      Files
      Additional Information
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      TRiP-OE
      (Transgenic RNAi Project members, 2017-)
      TRiP-OE-VPR
      (Zirin et al., 2020)
      Name Synonyms
      A set of transgenic sgRNA constructs for targeted gene overexpression by dCas9-VPR.
      (Transgenic RNAi Project members, 2017-)
      Secondary FlyBase IDs
        References (3)