A characterization of the diverse populations of cells in the entire body, head, and 15 dissected tissues of the adult Drosophila melanogaster.
Reads were processed using the Cell Ranger 3.1.0 software. They were aligned to the Drosophila melanogaster reference genome 6.31. Counts were corrected for the presence of contaminating ambient RNA using DecontX (R package Celda 1.4.5). Cells with less than 200 genes, less than 500 counts, or more than 5% of mitochondrial genes were excluded. Following dimensionality reduction and clustering, cell types were identified in annotation jamborees by tissue experts and annotations were gathered on the SCope platform.
The EMBL-EBI's Single Cell Expression Atlas provides cell-level annotations, clustering data, raw and normalised read counts, and putative marker genes.
