construct_comment: Described as "responder" construct. Allows cDNA of choice to be cloned downstream of tet operon operator sequences. Allows multifacted control: presence or absence of tTA activator (see pP{RHT}); presence or absence of tetracycline.
report comment: Carries 7 copies of Tn10\tetO operator repeat sequences, which are bound by Tn10\tetR ::Hsim\VP16 transactivator protein (tTA) in the absence of tetracycline.
construct_comment: Described as "responder" construct. Allows cDNA of choice to be cloned downstream of tet operon operator sequences. Allows multifacted control: presence or absence of tTA activator (see pP{RHT}); presence or absence of tetracycline.
report comment: Carries 7 copies of Tn10\tetO operator repeat sequences, which are bound by Tn10\tetR ::Hsim\VP16 transactivator protein (tTA) in the absence of tetracycline.