FB2024_03 , released June 25, 2024
Recombinant Construct: pP{WTP}
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General Information
Symbol
pP{WTP}
FlyBase ID
FBmc0002733
Feature type
engineered_plasmid
Component Allele(s)
Size
Expression Data
Associated insertion(s)
Molecular map
Description and Uses
Construct components
Sequence Data
Sequence (FB)
Associated Sequence Data
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Component Allele
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
Comments

construct_comment: Described as "responder" construct. Allows cDNA of choice to be cloned downstream of tet operon operator sequences. Allows multifacted control: presence or absence of tTA activator (see pP{RHT}); presence or absence of tetracycline.

report comment: Carries 7 copies of Tn10\tetO operator repeat sequences, which are bound by Tn10\tetR ::Hsim\VP16 transactivator protein (tTA) in the absence of tetracycline.

Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Secondary FlyBase IDs
    References (3)