From das_m_pradeep@XXXX Wed Jun 17  22:17:50  1998
Envelope-to: gm119@XXXX
Delivery-date: Wed, 17 Jun 1998  22:17:50  +0100
Date: 17 Jun 1998  17:27:12  -0500
From: 'Das, Pradeep' <das_m_pradeep@XXXX>
Subject: Re. FlyBase query
To: 'Gillian Millburn' <gm119@XXXX>
Cc: 'rick' <padgett@XXXX>
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Dear Gillian,
The tkv* construct used for the experiments described in our paper (see below)
is indeed downstream of UAS elements. However, the expression of the construct
was not driven by a GAL4 line, but rather by enhancer piracy, and hence the
promoter is unknown. The only information we are in a position to provide is
that the line used is a jump of UAS-tkv* onto the TM3, Sb balancer chromosome.
Please feel free to write to me for further clarifications.
Regards,
Pradeep Das
>Dear Dr. Padgett,
>
>I am curating your paper for FlyBase:
>
>Das et al., 1998, Development 125(8): 1519-1528
>
>and I have a question about the activated tkv* construct used in your
>paper.
>
>Is this construct expressed using UAS regulatory sequences, and if
>so, was expression driven using enGAL4 - as for the activated sax*
>construct. If the tkv* construct is not expressed under the control
>of UAS regulatory sequences, what promoter sequences drive its
>expression ?
>
>I look forward to hearing from you,
>
>Gillian Millburn
From gm119@XXXX Thu Jun 18  10:46:12  1998
Envelope-to: gm119@XXXX
Delivery-date: Thu, 18 Jun 1998  10:46:12  +0100
To: das'_m_pradeep@XXXX
Subject: Re: FlyBase query
Cc: gm119@XXXX
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From: Gillian Millburn (Genetics) <gm119@XXXX>
Date: Thu, 18 Jun 1998  10:56:19  +0100
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Dear Pradeep,
thanks for your reply, it was very helpful.
I would just like to confirm whether the UAS-tkv* construct used to make
your tkv* enhancer piracy line is the construct that was described in:
Lecuit et al., 1996, Nature 381(6581): 387--393
I would like to record the details of your tkv* enhancer piracy line as a
personal communication to FlyBase from you. Is that OK ?
Also, do you have a particular designation for the line, as this helps in
naming the construct in FlyBase.
Gillian
From das'_m_pradeep@XXXX Thu Jun 18  11:15:01  1998
Envelope-to: gm119@XXXX
Delivery-date: Thu, 18 Jun 1998  11:15:01  +0100
Date: 18 Jun 1998  06:24:51  -0500
From: 'Das, Pradeep' <das'_m_pradeep@XXXX>
Subject: RE: FlyBase query
To: 'Genetics' <gm119@XXXX>
Cc: 'rick' <padgett@XXXX>,
'Cho, Seo-Hee' <cho@XXXX>
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Dear Gillian,
the UAS-tkv* construct described in our paper was made in our lab, so while the
nature of the mutation may be the same as the one in Lecuit et al. (and in
several other papers), the transgenic line itself is unique. The designation we
use for this line is 'SC143' (TM3, Sb, tkv*).
Please cite personal communication from Seo-Hee Cho - he's the student in our
lab that made the lines and characterised them.
Hope that was helpful! Please feel free to email me for further clarifications.
Regards,
Pradeep
From gm119@XXXX Thu Jun 18  13:09:48  1998
Envelope-to: gm119@XXXX
Delivery-date: Thu, 18 Jun 1998  13:09:48  +0100
To: das'_m_pradeep@XXXX
Subject: RE: FlyBase query
Cc: padgettXXXX, choXXXX, gm119@XXXX
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From: Gillian Millburn (Genetics) <gm119@XXXX>
Date: Thu, 18 Jun 1998  13:19:48  +0100
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Dear Pradeep,
as you made the UAS-tkv* construct in your lab, I will make a new entry in
FlyBase for it.  Is the mutation Q199D, as described in the materials and
methods of your paper for Ubi-tkv ? and is it in the pUAST vector ?
As I've been writing to you, it seems fair if I cite the personal
communication from both Seo-Hee Cho and you, and I'll record that the
construct was made by him in the personal communication, is that OK ?
Sorry to keep e-mailing you, and thanks for being so helpful !
Gillian
From das'_m_pradeep@XXXX Thu Jun 18  15:04:33  1998
Envelope-to: gm119@XXXX
Delivery-date: Thu, 18 Jun 1998  15:04:33  +0100
Date: 18 Jun 1998  10:13:44  -0500
From: 'Das, Pradeep' <das'_m_pradeep@XXXX>
Subject: RE: FlyBase query
To: 'Genetics' <gm119@XXXX>
X-Mailer: Mail*Link SMTP-MS 3.0.2
Content-Length: 675
dear gillian,
>FlyBase for it.  Is the mutation Q199D, as described in the materials and
>methods of your paper for Ubi-tkv ? and is it in the pUAST vector ?
yes to both.
regards,
pradeep