From preiss@XXXX Fri Feb 18 08:25:10 2000 Envelope-to: cy200@XXXX Delivery-date: Fri, 18 Feb 2000 08:25:10 +0000 Date: Fri, 18 Feb 2000 09:29:02 +0100 (MEZ) From: Anette Preiss <preiss@XXXX> To: Chihiro Yamada <cy200@XXXX> Subject: Re: FlyBase Query (cy360) Dear Chihiro Yamada, hopefully the answers enclosed will be helpful. 1. gro alleles. These 5 alleles are described in Preiss, A., D. Hartley and S. Artavanis-Tsakonas (1988). The molecular genetics of Enhancer of split, a gene required for embryonic neural development in Drosophila. EMBO J. 7: 3917-3928 In Lindsley and Zimm (1992), they were renamed r17 - r22, however, these names are not commonly used in the literature. All five are EMS induced alleles in an e4 tx background. They uncover the recessive phenotype of gro1 and are lethal in trans and over deficiencies. Therefore, they were named alleles of l(3) gro. The mutations all affect the transcription unit m9/m10 in the E(spl) region because they can be rescued with a respective trans-gene. I am not aware that any of these alleles affect the neighboring transcription unit E(spl) bHLH m8 and it seems rather unlikely because they do not show any obvious molecular or cytological aberrations. However, as long as there are no sequence data of these alleles available, a double hit in m8 cannot be excluded. As far as I can tell, mutations in m8 do not give any apparent phenotype. Therefore, a separation of mutations in the two genes can only occur at the molecular level. There is some confusion in naming the genes in the E(spl) region which is based on the problems in their original characterization. Two 'historical' alleles, gro and E(spl) have been name giving. E(spl) is a mutation in m8 which has little apparent phenotype on its own but enhances the eye roughening of the recessive split allele of Notch in a dominant manner (thus: E(spl)D). Revertants of E(spl)D were generated that are mostly structural mutations (deletions and translocations) which affect several genes in the region. Nearly all of them affect the neighboring gro gene with exception of the three mutations mentioned in the paper. This is why the name E(spl) was extended towards the whole region and the respective genes that are further identified by their transcription unit label (m8 - mdelta). .. Best regards, Anette Preiss \---------------------------------------------------------------------- Prof. Dr. Anette Preiss University of Hohenheim Department of Genetics (240) Ph: ++49-711-459 2206 70593 Stuttgart FAX: ++49-711-459 2211 F.R. Germany e-mail: preiss@XXXX \---------------------------------------------------------------------- From cy200 Wed Feb 16 12:04:56 2000 To: preiss@XXXX Subject: FlyBase Query (cy360) Cc: me X-Sun-Charset: US-ASCII Content-Length: 1610 Dear Dr Preiss, I am currently curating your paper for FlyBase: Nagel et al., 1999, Dev. Genet. 25(2): 168--179 I have a few questions I was hoping you could help me with. 1. gro alleles. In your materials and methods section, you mention a number of gro alleles: E28, E48, E73, E75, E105. Looking through our records, we don't have all of these as alleles of gro, some of these appear as alleles of m8. Are these all definitely alleles of gro? Are they also alleles of m8? If they are alleles of both, are the gro and m8 phenotypes known to be separable? It is not clear from our records what the situation is for these alleles and it would be very helpful if you could help me sort this out. .. Any information you give would be entered as a 'personal communication to FlyBase from you to FlyBase, provided you are happy with that. Thanks and Best wishes, Chihiro \------------------------------------ Chihiro Yamada. FlyBase (Cambridge), Department of Genetics, University of Cambridge, Downing Street, email: c.yamada@XXXX Cambridge, CB2 3EH, Ph : 01223-333963 UK. FAX: 01223-333992 \----------------------------------------------------------------------
