From yhiromi@XXXX Thu Aug 17 11:42:06 2000 Envelope-to: cy200@XXXX Delivery-date: Thu, 17 Aug 2000 11:42:06 \+0100 Mime-Version: 1.0 X-Mailer: Macintosh Eudora Pro Version 4.0.1Jr1 Date: Thu, 17 Aug 2000 19:48:30 \+0900 To: Chihiro Yamada <cy200@XXXX> From: Yasushi Hiromi <yhiromi@XXXX> Subject: Re: FlyBase Query (cy735b) Dear Dr. Yamada, As Dr. Lu states, I provided the B38 strain to him. Both B38 and H214 are enhancer trap insertions into the klingon gene (1). However, they are not the same stock; B38 has an insertion of the plwB element (2), whereas H214 (3) carries the HZ element (4). B38 was generated in an enhancer trap screen done in Corey Goodman's laboratory, and we (Samantha Butler and Yasushi Hiromi) identified that it is an insertion in the klingon gene. Although we have not published about B38, we are happy to share the stock and the information with the fly community. It can be referred to as 'S. Butler and Y. Hiromi, personal communication'. 1. Butler SJ, Ray S, Hiromi Y (1997). klingon, a novel member of the Drosophila immunoglobulin superfamily, is required for the development of the R7 photoreceptor neuron. Development 124(4):781-92. 2. The reference is either Genes Dev. 1989 Sep;3(9):1301-13 or Bioessays. 1990 May;12(5):199-204. 3. Mlodzik M, Hiromi Y, Goodman CS, Rubin GM (1992). The presumptive R7 cell of the developing Drosophila eye receives positional information independent of sevenless, boss and sina. Mech Dev 37(1-2):37-42. 4. Mlodzik M, Hiromi Y, Weber U, Goodman CS, Rubin GM (1990). The Drosophila seven-up gene, a member of the steroid receptor gene superfamily, controls photoreceptor cell fates. Cell 60(2):211-24. I hope the information is useful to you. Sincerely, Yash Hiromi At 10:28 \+0100 00/08/17, Chihiro Yamada wrote: > Dear Dr Hiromi, > > I am currently curating for FlyBase: > > Zhang et al., 2000, Mech. Dev 95(1,2): 113--122 > > Having recently corresponded with Dr Xiangyi Lu, he suggested that I > talk to you about one of the questions I had relating to the paper > above. This is the question I was hoping you could answer for me. > > \--- > > I wrote: > > B38-lacZ > > ======== > > In your Experimental Procedures section you mention B38-lacZ. You > > state that it is 'allelic to H214-lacZ'. I'm not sure what you mean by > > this. Are the B38-lacZ and H214-lacZ are caused by the same insertion? > > Are they two identical insertions? Are they both insertions of the > > same transposon? I'd appreciate any information you could give about > > the relationship between B38-lacZ and H214-lacZ that might help me sort > > this out. > > Dr Lu wrote: > B38-lacZ WAS FROM Y. YIROMI'S UNPUBLISHED STOCK. WHAT I HAVE CITED IN THE >PAPER > IS WANT YIROMI COMMUNICATED TO ME. I DON'T HAVE A COPY OF THE PAPER IN FRONT >OF > ME SINCE ALL MY PAPERS HAVE BEEN SHIPPED TO BIRMINGHAM WHERE I AM MOVING TO >THIS > MONTH. THE BEST PERSON TO ASK IS DR. YIROMI. > > \--- > > If you could find the time to answer this question I would greatly > appreciate it. > > Best wishes, > > Chihiro > > \---------------------------------------------------------------------- > Chihiro Yamada. > > FlyBase (Cambridge), > Department of Genetics, > University of Cambridge, > Downing Street, email: c.yamada@XXXX > Cambridge, CB2 3EH, Ph: 01223-333963 > UK. FAX: 01223-333992 > \---------------------------------------------------------------------- Yasushi Hiromi National Institute of Genetics 1111 Yata, Mishima Shizuoka 411-8540, JAPAN phone: \+81-559-81-6767 FAX: \+81-559-81-6768 e-mail: yhiromi@XXXX
