To: Chihiro Yamada <cy200@XXXX> From: Daniel St Johnston <ds139@XXXX (Daniel St. Johnston)> Subject: Re: FlyBase Query (cy1359) Date: Fri, 24 Aug 2001 12:08:18 \+0100 Hi chihiro, . The lethality associated with P{PZ}rL203 is not due to the insertion, and Freek recombined it off before mobilising the P. . Dr Daniel St Johnston Wellcome/CRC Institute Tennis Court Rd Cambridge CB2 1QR United Kingdom Tel: (0)1223-334-127 Fax: (0)1223-334-089 To: ds139@XXXX Subject: FlyBase Query (cy1359) From: Chihiro Yamada <cy200@XXXX> Date: Fri, 24 Aug 2001 11:26:20 \+0100 Hi Daniel! I'm currently curating your paper for FlyBase: van Eeden et al., 2001, J. Cell Biol. 154(3): 511--524 P{PZ}rL203 ========== This insertion is in the 5'UTR of btz. On page 515 you tell us that it 'does not cause a phenotype'. Does this mean that the homozygous insertion does not cause a btz phenotype or does not cause any phenotype at all, and is thus wild-type? I need to know because currently this insertion is in our records as causing an allele l(3)rL203rL203, which is stated to be homozygous lethal. If there is a real discrepancy between the phenotypes here then it suggests the lethal phenotype is separable from the insertion and will need some extra sorting out. . Thanks Chihiro \---------------------------------------------------------------------- Chihiro Yamada. FlyBase (Cambridge), Department of Genetics, University of Cambridge, Downing Street, email: c.yamada@XXXX Cambridge, CB2 3EH, Ph : 01223-333963 UK. FAX: 01223-333992 \----------------------------------------------------------------------