Date: Thu, 06 Dec 2001 13:27:21 \-0500 To: flybase-updates@XXXX From: Kevin Cook <kcook@XXXX> Subject: Isolation and characterization of Df(3L)BSC8 Isolation and characterization of Df(3L)BSC8 Jennifer Deal and Kevin Cook Bloomington Stock Center Df(2L)BSC8 was isolated as a P transposase-induced male recombination event involving P{lacW}l(3)j11B2j11B2 and P{PZ}Eip75B07041. The deletion was isolated as a rho+-e+ recombinant chromosome from the cross rhove-1 pp e1 females x CyO, H{PDelta2-3}HoP2.1/+; rhove-1 P{PZ}Eip75B07041/P{lacW}l(3)j11B2j11B2 e1 males. Polytene chromosome squashes showed the breakpoints 74D3-75A1;75B2-5. Df(2L)BSC8 failed to complement Eip74EFDL-1. __________________________________________________________ Kevin Cook, Ph.D. Bloomington Drosophila Stock Center Department of Biology http://flystocks.bio.indiana.edu Jordan Hall 142 Indiana University 812-855-5782 1001 E. Third St. 812-855-2577 (fax) Bloomington, IN 47405-3700 kcook@XXXX