From kcook@XXXX Thu Dec 20 19:51:22 2001 To: flybase-updates@XXXX Subject: Isolation and characterization of Df(2L)BSC9 Isolation and characterization of Df(2L)BSC9 Jennifer Deal and Kevin Cook Bloomington Stock Center Df(2L)BSC9 was isolated as a P transposase-induced male recombination event involving P{PZ}cup01355 and P{lacW}l(2)k13315k13315. The deletion was isolated as a dp+-cn+ recombinant chromosome from the cross dpov1 cn1 bw1 females x dpov1 P{lacW}l(2)k13315k13315/P{PZ}cup01355 cn1; TMS, P{Delta2-3}99B/+ males. The deficiency chromosome retains the miniwhite marker from the P{lacW} element based on w+ eye color in a w1118 background. Polytene chromosome squashes showed the breakpoints 26F5-7;27A2-B2 (at least part of 27B2 remains as a faint band). The deletion fails to complement cortQW55. __________________________________________________________ Kevin Cook, Ph.D. Bloomington Drosophila Stock Center Department of Biology http://flystocks.bio.indiana.edu Jordan Hall 142 Indiana University 812-855-5782 1001 E. Third St. 812-855-2577 (fax) Bloomington, IN 47405-3700 kcook@XXXX