From kcook@XXXX Mon Apr 01 18:42:12 2002 To: flybase-updates@XXXX Subject: Isolation and characterization of Df(2L)BSC18 Isolation and characterization of Df(2L)BSC18 Megan Deal-Herr and Kevin Cook Bloomington Stock Center, Indiana University Df(2R)BSC18 was isolated as a P transposase-induced male recombination event involving P{lacW}mamk02214 and P{EP}EP993. The deletion was isolated as a cn+-bw+ recombinant chromosome from the cross cn1 bw1 females X cn1 P{EP}EP993/P{lacW}mamk02214 bw1 sp1; TMS, Sb1 P{Delta2-3}99B/+ males. Polytene chromosome squashes showed the breakpoints 50D1;50D2-7. Df(2R)BSC18 failed to complement cg1. __________________________________________________________ Kevin Cook, Ph.D. Bloomington Drosophila Stock Center Department of Biology http://flystocks.bio.indiana.edu Jordan Hall 142 Indiana University 812-855-5782 1001 E. Third St. 812-855-2577 (fax) Bloomington, IN 47405-3700 kcook@XXXX