Date: Mon, 28 Jul 2003 09:50:32 \-0700 To: Chihiro Yamada <cy200@XXXX> From: Ed Giniger <eginiger@XXXX> Subject: Re: FlyBase Query (cy1992) >Dear Dr Giniger, > >I am currently curating your paper for FlyBase: > >Crowner et al. 2003, Curr. Biol. 13(11): 967--972 > >I have a few questions I was hoping you could answer for me: > >UAS-Dl >====== the UAS-Dl is the one used in the Doherty paper (what the paper neglects to mention is that I'm the one who made it and gave it to them) >You use a UAS-Dl construct. We have 4 of these constructs in our >records \- is it one of these? > >DlScer\UAS.cDa > Doherty et al., 1996, Genes Dev. 10(4): 421--434 > Okajima and Irvine, 2002, Cell 111(6): 893--904 > Panin et al., 1997, Nature 387(6636): 908--912 > >DlScer\UAS.cHa > Jonsson and Knust, 1996, Dev. Genes Evol. 206(2): 91--101 > Klein et al., 1997, Dev. Biol. 189(1): 123--134 > Fanto and Mlodzik, 1999, Nature 397(6719): 523--526 > >DlScer\UAS.cJa > Baonza and Freeman, 2001, Development 128(20): 3889--3898 > Hukriede et al., 1997, Development 124(17): 3427--3437 > Lear et al., 1999, Mech. Dev. 88(2): 207--219 > >DlScer\UAS.cLa > Lee et al., 2000, Curr. Biol. 10(15): 931--934 > Li and Baker, 2001, Curr. Biol. 11(5): 330--338 > >If you are not sure, can you give me some more information on the one >that you used to help me identify it? Where did you get the line from? >Has it been published before? If so where? > >nrtM54 >======== the source of nrtM54 is discussed in the text. The mutant that has traditionally been called dabM54 (from Gertler and Hoffmann) is actually a mutation in nrt, as was shown recently by Liebl et al (Development, 2003; also referenced in the text) >We do not have a nrt allele with this name. Can you give me some more >information on this allele? Where did you get the line from? Has it >been published before? If so where and under what name? If its a new >allele can you give me some details? What was the mutagen used to make >this allele? What are the molecular details of this allele? > >trio123.4 >=========== trio 123.4 is from Eric Liebl. What he told me is that it is an imprecise excision of the P-element in l(3)036810 >We do not have a trio allele with this name. Can you give me some more >information on this allele? Where did you get the line from? Has it >been published before? If so where and under what name? If its a new >allele can you give mesome details? What was the mutagen used to make >this allele? What are the molecular details of this allele? > >sim-GAL4 >======== >You use a sim-GAL4 line in your paper. We have 2 of these constructs in our >records \- is it one of these? We got sim-GAL4 from Steve Crews, so my guess would be that it is the P3.7, but that is just a guess. > >Scer\GAL4sim.P3.7 > Battye et al., 1999, Development 126(11): 2475--2481 > Georgiou and Tear, 2002, Development 129(12): 2947--2956 > Kidd et al., 1998, Neuron 20(1): 25--33 > Kramer et al., 2001, Science 292(5517): 737--740 > Lanoue and Jacobs, 1999, Dev. Genet. 25(4): 321--330 > Noordermeer et al., 1998, Neuron 21(5): 991--1001 > Sedaghat et al., 2002, Development 129(11): 2591--2606 > Xiao et al., 1996, Mech. Dev. 58(1-2): 65--74 > Scer\GAL4 gene expression is driven by 3.7kb BamHI fragment of > sim 5' flanking DNA (and 18 amino acids of coding sequence) > containing the early sim promoter and 5' flanking sequences. > >Scer\GAL4sim.PS > Becker et al., 1997, Development 124(13): 2615--2622 > Bossing and Brand, 2002, Development 129(18): 4205--4218 > Dorfman et al., 2002, Dev. Biol. 252(1): 119--126 > Golembo et al., 1996, Development 122(11): 3363--3370 > Hummel et al., 2000, Neuron 26(2): 357--370 > Luer et al., 1997, Development 124(14): 2681--2690 > Sanchez Soriano and Russell, 1998, Development 125(20): 3989--3996 > Scholz et al., 1997, Mech. Dev. 62(1): 79--91 > Vorbruggen and Jackle, 1997, Proc. Natl. Acad. Sci. USA >94(16): 8606--8611 > 3.5kb EcoRV sim promoter fragment drives expression of > Scer\GAL4. > >If you are not sure, can you give me some more information on the one >that you used to help me identify it? Where did you get the line from? >Has it been published before? If so where? > >Any new information you give me will be curated as a personal >communication from you to FlyBase, if thats fine with you. Feel free to quote me as a personal communication for any of the information above. >Best wishes, > >Chihiro > >---------------------------------------------------------------------- >Chihiro Yamada. > >FlyBase (Cambridge), http://fbserver.gen.cam.ac. uk:7081 / >Department of Genetics, >University of Cambridge, email: c.yamadagen.cam.ac.uk >Downing Street, Tel : 01223-333963 >Cambridge, CB2 3EH, FAX : 01223-333992 >United Kingdom. Memes don't exist. Spread the Word. >----------------------------------------------------------------------