From kcook@XXXX Fri Aug 29 20:57:30 2003 To: flybase-updates@XXXX Subject: Isolation and Characterization of Df(2R)BSC40 Isolation and Characterization of Df(2R)BSC40 Megan Deal-Herr and Kevin Cook Bloomington Stock Center, Indiana University Df(2R)BSC40 was isolated as a P transposase-induced male recombination event involving P{GT1}Rep1BG01033 and P{PZ}Cct506444. The deletion was isolated as a cn+-bw+ recombinant chromosome from the cross cn1 bw1 females x cn1 P{PZ}Cct506444/ P{GT1}Rep1BG01033 bw1 sp1; TMS, Sb1 P{ry+t7.2=Delta2-3}99B/+ males. Polytene chromosome squashes showed the breakpoints 48E1-2;48E2-10. Df(2R)BSC40 failed to complement SmD3k09029 and jebk05644. __________________________________________________________ Kevin Cook, Ph.D. Bloomington Drosophila Stock Center Department of Biology http://flystocks.bio.indiana.edu Jordan Hall 142 Indiana University 812-855-5782 1001 E. Third St. 812-855-2577 (fax) Bloomington, IN 47405-3700 kcook@XXXX