From ermunoz@XXXX Mon Jan 05  12:59:56  2004
To: rd120@XXXX
Subject: Drosophila II...
Dear Rachel,
Please find attached the file with information
on Group D lethals.
Best regards. Enzo
\--------------------------------------------------------------------------
GROUP D LETHALS
Though not stated in each case, all lethals are covered by the Dp (1;Y) mal+.
(Fb): Flybase
\-Df(1) n20 (Fb), (Syn. 17-274)
\-Df(1)17-148 (Fb) and
\-Df (1)17-441
have all similar break points limits. The three are lethal with Df(1)n23 (Fb),
(Syn.
Df(1)17-123) and viable with Df(1)n49 (Syn. Df(1)17-439 (Fb)).
The data on the Flybase under _______n49____
'Genetic data about other abe.'
(17-439 and n20) needs to be
corrected according to this scheme ____n23_____
*_ n20, 17-148,
17-441_
Break points in Fb: O.K. Additional information:
Lethal with Dfs. B-111 and Df(1)17-19 and with lethals Q-463, x-4, R-9-18,
3-Des.
Viable with Dfs. B-57, B-12, 16-3-35 (Fb), 17-25 (Fb) and 17-252 (Fb) and with
lethals A-200, Q-56, Q-456, 114, x-3, P-19, Q-463. Normal with bbl.
Df(1)18-76
Lethal with Df(1) B-111 and with lethals A-200, Q-56, Q-456 and 114. Viable
with Dfs. B-57, B-12, 16-3-35 (Fb), 17-25 (Fb), n23 (Fb) and with lethals x-3,
P-
19, Q-463, x-4, R-8-18, 3-Des, E-54 and 14-1. Df(1)18-76/Df(1)n23 or /Df(1)18-
61 or /Df(1)16-1-79 or /Df (1)16-2-3, shows visible (abnormal wings, veins
and
bristles, ret ?). Normal with bbl
bk 1: Between eo and wap. Left limit from non-inclusion of l (1)E-54; right from
inclusion of A-200
bk 2: Between l(1)114 (fog) and l(1)x-3 (stnA). Left limit from inclusion of
ll4 (and
ret ?); right from non-inclusion of x-3.
_________18-76_________ret ?
......._____n23____
......._____18-61_____________
......._____16-1-79_______
......._____16-2-3________
___________________________________________________________________
Df(1)18-61
Not covered by Dp (1;Y) BS Y y+. Lethal with Df (1)B-111 and with lethals
x-3, P-
19, Q-463, x-4, R-9-18, 3-Des, bbl. Viable with lethals A-200, Q-56, Q-456, 114
and 16-3-190 and with Dfs. B-57, B-12, 16-3-35 (Fb), 17-25 (Fb). With Dfs, 18-76
and 17-454 shows visible (see 18-76)
bk1: Between l (1)114 and l (1)x3. Left limit from non-inclusion of 114;
right from
inclusion of x-3 (and ret ? ).
bk 2: To the right of bb
___________________________________________________________________
Df(1)16-1-79 and 16-2-3
Both are male sterile. Lethal with Df(1)B-111 and with lethals x-3, P-19,
Q-463, x-
4, R-9-18, 3-Des. Viable with Dfs. B-57, B-12, 16-3-35 (Fb), 17-25 (Fb), l7-466
(Fb) and 18-76 (it shows visible, see l8-76), and with lethals A-200, Q-56,
Q-456,
114. Df(1)16-1-79 has normal phenotype with bbl; Df(1)16-2-3 was not tested with
bbl
bk1: Between l(1)114 and l (1)x3. Left limit from non-inclusion of 114; right
from
inclusion of x-3 (and ret ?)
bk2: Df(1)16-1-79 : Between l(1)3-Des (su(f)) and bb. Left limit from
inclusion of
3-Des; right from non inclusion of bb.
Df(1)16-2-3: To the right of su(f) from inclusion of 3-Des .
___________________________________________________________________
Df(1)18-74
Covered by Dp(1;Y)BS Y y+ . Male sterile. Lethal with Df (1)B-111 and with
lethals Q-463, x-4. Viable with Dfs. B-57, B-12, 16-3-35 (Fb), l7-25 (Fb),
16-185
(Fb), 17-252 (Fb) and with lethals A-200, Q-56, Q-456, 114, x-3, P-19, R-9-18,
3-
Des. Normal with bbl.
bk1: Between l(1)P-19 (20 Ca) and Q-463 (20 Cb). Left limit from non-inclusion
of
P-19; right from inclusion of Q-463.
bk2: Between x-4 (sph) and l (1)R-9-18. Left limit from inclusion of x-4;
right from
non-inclusion of R-9-18.
_________________________________________________________________Df
(1)17-454
Not covered by Dp (1;Y) BS Y y+. Male sterile. Lethal with Df(1)B-111 and with
l(1)114. Viable with Dfs. B-57, B-12, 16-3-35 (Fb), l7-25 (Fb), 16-1-80 and
n23
(it shows visible, ret ? see Df(1)18-76) and with lethals A-200, Q-56,
Q-456, x-3,
P-19, Q-463, x-4, R-9-18, 3-Des and l6-1-49. Normal with bbl
bk1: Between l(1)Q-456 (uncl) and l(1)114. Left limit from non-inclusion of Q-
456; right from inclusion of 114.
bk2: Between 114 (or ret ?) and x-3 . Left limit from inclusion of ret ?;
right from
non-inclusion of x-3.
__________________________________________________________________
Df(1)17-19
Not covered by Dp (1;Y)BS Y y+. Lethal with Df(1)B-111 and with lethals 114, x-
3, P-19, Q-463, 16-3-190. Viable with Dfs. B-57, B-12, 16-3-35 (Fb), 17-25 (Fb),
16-1-80 and 17-87 and with lethals A-200, Q-56, Q-456, x-4, R-9-18 and 3-Des.
Normal with bbl
bk1: Between l(1)Q-456 (uncl) and l(1)114 (fog). Left limit from non-inclusion
of
Q-456; right from inclusion of 114.
bk2: Between l(1)Q-463 (20Cb) and x-4 (sph). Left limit from inclusion of Q-463;
right from non inclusion of x-4.
__________________________________________________________________
Df (1)17-401
Not covered by Dp (1;Y)BS Y y+. Lethal with Df (1)B-111 and with lethals 114, x-
3, P-19, Q-463, x-4, R-9-18, 3-Des. Viable with Dfs. B-57, B-12, 16-3-35 (Fb),
17-
25 (Fb) and 16-1-80 and with lethals A-200, Q-56 and Q-456. Normal with bbl.
bk1: Between uncl and fog . Left limit from non-inclusion of l (1) Q-456; right
from inclusion of l (1) 114.
bk2: Between l (1)3-Des (su(f)) and bb. Left limit from inclusion of 3-Des;
right
from non-inclusion of bb.
__________________________________________________________________
Df(1)17-228 and Df(1)17-369
Break point similar to Df (1) 17-137 already described in the Flybase.
Additional
information: Lethal with Dfs. B-111, B-12 and with lethals A-200, Q-56, Q-456,
114, x-3, P-19, Q-463, x-4, R-9-18, 3-Des, E-54 and 14-1. Viable with Dfs. B-57,
16-3-35 (Fb), 17-25 (Fb), 16-1-29 (Fb) and 18-80 (Fb) and with lethals B-56, B-
214, w-2, w-4, LB20, 17-62 (Fb). Normal with ot and with bbl bk1: Between l
(1)A-112 (Hlc) and l (1)E-54 (eo). Left limit from non-inclusion of A-112, right
from inclusion of E-54.
bk2: Between su(f) and bb. Left limit from inclusion of 3-Des, right from non-
inclusion of bb.
Df(1)17-412
Break points similar to Df(1)17-59 already described in the Flybase. The only
difference is that Df (1)17-412/Dp (1;Y) mal+ males are sterile.
Additional information: Lethal with Dfs. B-111 and B-12 and with lethalsA-200,
Q-56, Q-456, 114, x-3, P-19, Q-463, x-4, R-9-18, 3-Des, LB20, 17-62 (Fb) and
14-
1. Viable with Dfs. B-57, 16-3-35, 17-25, 16-129 (Fb), 18-80 (Fb) and with
lethals
B-56, B-214, w-2, w-4, 16-3-212 (Fb). Normal with ot and with bbl.
bk1: Between l (1)w-4 (l (1)19-Fe4) and LB20.Left limit from non-inclusion of w-
4, right from inclusion of LB20.
bk2: Between su(f) and bb. Left limit from inclusion of l (1) 3-Des, right
from non-
inclusion of bb
___________________________________________________________________
Df(1)16-1-88
Lethal with Dfs. B-111, B-57, B-12, A-118 and 16-3-35 and with lethals A-200, Q-
56, Q-456, 114, x-3, P-19, Q-463, x-4, R-9-18, 3-Des, B-56, B-214, w-2, w-4,
LB20, 17-62 (Fb), 14-1, 16-3-212 (Fb), , R-9-28, E-81 and bbl. Viable with Dfs.
17-25 (Fb) and mal-10 and with l (1)AA-33. Normal with ot. Df (1)16-1-88/Dp
(1;Y) mal+ males are sterile.
bk1: Between l (1)AA-33 (run, leg) and l(1) E-81. Left limit from
non-inclusion of
AA-33 and mal-10, right from inclusion of l (1) E-81.
bk2: To the right of bb ?. Left limit from the inclusion of 3-Des; right from
lethality
with bbl.
___________________________________________________________________
Df (1)19-2
Break Points similar to Df (1) 17-489 already described in the Flybase.
Additional
information: Df (1)19-2/Dp(1;Y)mal+ males are sterile. Lethal with Dfs. B-111,
B-
57, A-118, B-12 and T2-14A and with lethals A-200, Q-56, Q-456, 114, x-3, P-19,
Q-463, x-4, R-9-18, 3-Des, B-56, B-214, w-2, w-4, LB20, l7-62 (Fb), 14-1, 16-3-
212 (Fb) and bbl.
Viable with Dfs. 16-3-35 (Fb) and 17-25 (Fb) and with lethals R-9-28, E-81 and
AA-33.
Normal with ot.
bk1: Fb
bk 2: Fb
Df (1) 19-18 and Df (1) 16-3-129
Lethal with Dfs. B-111, B-57, A-118, B-12 and 16-3-35 and with lethals A-200, Q-
56, Q-456, 114, x-3, P-19, Q-463, x-4, R-9-18, 3-Des, B-56, B-214, w-2, w-4,
LB20, 17-62 (Fb), 14-1, 16-3-212 (Fb), , R-9-28, E-81, AA-33 and bbl. Viable
with
Dfs. 17-25 (Fb) and mal-10. Normal with ot. Df (1)19-18/Dp (1;Y) mal+ males are
sterile.
bk1: Between mell and l(1)AA-33. Left limit from viability with Df (1) 17-25;
right
from inclusion of AA-33.
bk2: Left limit from inclusion of l (1)3-Des (su(f)); right to the right of bb
?, from
lethality with bbl.
___________________________________________________________________
Df (1) 16-3-139
Lethal with Dfs. B-111, B-57, B-12, 16-3-35 and T2-14 A and with lethals A-200,
Q-56, Q-456, 114, B-56, B-214, w-2, w-4, LB20, 17-62 (Fb), 14-1, 16-3-212 (Fb),
E-81 and AA-33. Viable with Dfs. 17-25 (Fb), n20 (Fb) and mal-10 and with
lethals x-3, P-19, Q-463, x-4, R-9-18, 3-Des. With Dfs. n24 and n23 (Fb) it
shows
visible, ret ? . Df (1)16-3-139/Dp (1;Y) mal+ males are sterile.
bk1: Between mell and l (1)AA-33. Left limit from viability with Df (1) 17-25;
right from inclusion of AA-33.
bk2: Between ret ? and x-3 (stnA). Left limit from inclusion of ret; right
from non-
inclusion of x-3.
Df (1)17-244
Lethal with Dfs. B-111, B-57 and B-12 and with lethals A-200, Q-56, Q-456, 114,
x-3, P-19, Q-463, x-4, R-9-18, 3-Des, B-56, B-214, w-2, w-4, LB20, 17-62 (Fb),
14-1, 16-3-212 (Fb) Viable with Dfs. 16-3-35 (Fb), 17-25 (Fb) and A-118 and with
l (1) 17-169 (Fb). The latter has two separate lethals, one is allelic to run
(run19)
and the other to vao . Normal with ot and with bbl
bk1: Between vao and unc. Left limit from non-inclusion of l (1)17-169 and Df
(1)
A-118; right from inclusion of l(1) 16-3-212.
bk2: Between su(f) and bb. Left limit from inclusion of 3-Des; right from non-
inclusion of bb
Df (1) 18-36
Lethal with Dfs. B-111, B-57, B-12, 16-3-35 (Fb), 17-25 Fb), A-118, T2-4A and
16-2-5 and with lethals A-200, Q-56, Q-456, 114, x-3, P-19, Q-463, x-4,
R-9-18, 3-
Des, B-56, B-214, w-2, w-4, LB20, 17-62 (Fb), 14-1, 16-3-212 (Fb), E-54, A-112,
w-1, R-9-28, E-81, AA-33, 19Cc, 16-3-98 (Fb), 16-1-27 (Fb), 17-234 (Fb). Visible
with ot, bbl and mal.
bk1: To the left of ot, from inclusion of ot.
bk2: disrupt bb
___________________________________________________________________
Df (1)17-322
Lethal with Dfs. B-12, B-57, 16-3-35 (Fb), 17-25 (Fb) and 17-137 (Fb) and with
lethals E-54, A-112, LB20, w-4, w-2, B-214, B-56, w-1, A-118, R-9-28, E-81 and
AA-33. Viable with Dfs. B-111, 16-2-14 and T2-4A and with lethals 19Cc and 16-
3-98 (Fb). Normal with ot and bbl . Shows mell and mal with Df (1) mal10 .
bk1: Between l (1) 19Cc and mal. Left limit from non-inclusion of 19Cc; right
from
inclusion of mal.
bk2: Between eo and wap. Left limit from inclusion of E-54; right from non-
inclusion of Dfs. B111 and 16-2-14.
___________________________________________________________________
Df (1) 16-3-95
Lethal with Dfs. B-12, B-57, 16-3-35 (Fb) A-118 and 17-137 (Fb) and with lethals
E-54, A-112, LB20, w-4, w-2, B-214, B-56, w-1, R-9-28, E-81 and AA-33. Viable
with Dfs. B-111, mal-10, 17-25 (Fb) and 16-2-14 . Normal with ot and bbl.
bk1: Between mell and run. Left limit from non inclusion of Df (1) 17-25; right
from inclusion of AA-33.
bk2: Between eo and wap. Left limit from inclusion of E-54; right from non-
inclusion of Dfs. B-111 and 16-2-14.
___________________________________________________________________
Df (1)16-3-160
Lethal with Dfs. B-12, B-57, A-118, 16-3-35 and 17-137 (Fb) and with lethals E-
54, A-112, LB20, w-4, w-2, B-214, B-56, w-1, R-9-28 and E-81. Viable with Dfs.
B-111, 17-25 (Fb), 16-2-14 and mal-10 and with lethals AA-33 and 17-26 (Fb).
Normal with bbl and ot.
bk1: Between run and l (1) E-81. Left limit fron non-inclusion of l (1) AA-33;
right
from inclusion of E-81.
bk2: Between eo and wap. Left limit from inclusion of E-54; right from non-
inclusion of Dfs. B-111 and 16-2-14.
___________________________________________________________________
Df (1)16-3-53 and Df (1) 17-456
Lethal with Dfs. B-12, B-57 and 16-3-35 and with lethals E-81 and AA-33. Viable
with Dfs. mal-10, 17-351 (Fb), A-118, 17-137 (Fb), 17-25 (Fb), 16-2-14 and B-111
and with lethals E-54, A-112, LB20, w-4, w-2, B-214, B-56, w-1 and R-9-28.
Normal with bbl and ot. Df (1) 16-3-53 and Df (1) 17-456/Dp (1;Y) mal+ males are
sterile.
bk1: Between mell and run. Left limit from non-inclusion of Df (1) 17-25; right
from inclusion of l (1) AA-33.
bk2: Between l (1) E-81 and l (1) R-9-28. Left limit from inclusion of E-81;
right
from non-inclusion of R-9-28.
___________________________________________________________________
Df (1) 16-2-5
Df (1) 16-2-5/Dp (1;Y) mal \+ males are sterile. Lethal with Df (1) 17-25 (Fb).
Viable with Dfs. B-111, B-12, 16-3-35 (Fb), 17-322 and T2-4A and with lethals
AA-33, 16-1-27 (Fb), 17-234 (Fb). Not tested with l (1) 19 Cc. Not tested with
l(1) 16-3-98 (Fb) (19-Cb1) because in both cases the males are sterile. Do not
show
mel in combination with Df (1) T2-4A . Do not show mal with Df (1) 17-322.
bk1: Left limit from non-inclusion of mel; right undetermined.
bk2: Left limit undetermined, right from non inclusion of mal.
___________________________________________________________________
l (1) 16-1-49
Lethal with Dfs. B-111 and 17-439 (Fb) and with l (1) x-3. Viable with Dfs.
B-57,
B-12, 16-3-35 (Fb), 17-25 (Fb), 17-274 (Fb) and 18-76 and with lethals A-200, Q-
56, Q-456, 114, P-19, Q-463, x-4, R-9-18, 3-Des. Normal with bbl. Covered by Dp
(1;Y) BS Y y+
Location: Allele of x-3 (stnA).
___________________________________________________________________
l (1) 16-3-4 and l (1) 17-410
Lethal with Dfs. B-111 and 16-3-34 and with l (1) Q-56. Viable with Dfs. B-57,
B-
12, 16-3-35 (Fb), 17-25 (Fb) and 17-196, and with lethals A-200, Q-456, 114,
x-3,
P-19, Q-463, x-4, R-9-18 and 3-Des. Normal with bbl. Not covered by Dp (1;Y)
BS Y y+. Both are male sterile with Dp (1;Y) mal+.
Location: Male sterile alleles of l (1) Q-56 (intro ?)
l (1) 17-344
Lethal with Dfs. B-12, B-57 and A-118 and with lethal R-9-28. Viable with Dfs.
B-
111, 16-3-35 (Fb), 17-25 (Fb), 17-137 (Fb), 16-2-14 and T2-14A. and with lethals
E-54, A-112, LB20, w-4, w-2, B-214, B-56, w-1, E-81 and AA-33. Normal with ot
and with bbl.
Location: Allele of l (1) R-9-28.
l (1) 16-2-9
Lethal with Dfs. B-111 and 16-3-34 and with lethals Q-56 and x-4. Viable with
Dfs. B-57, B-12, 16-3-35 (Fb), 17-25 (Fb), 16-1-85 (Fb), 17-2-52 (Fb) and 17-19
and with lethals A-200, Q-456, 114, x-3, P-19, Q-463, R-9-18 and 3-Des. Normal
with bbl.
Location: Is a double lethal, one is allelic to l (1) Q-56 and the other to l
(1) x-4
(sph).
From ermunoz@XXXX Mon Jan 19  12:44:30  2004
To: rd120@XXXX
Subject: Drosophila Df(1)17-25
Dear Rachel,
I had, the apparently wrong idea, that Df(1)17-25
had been previously described in the Flybase,
but when checking if somebody had done the
cytology I couldn't find it in the FlyBase. Since I used it
for mapping other deficiencies I think it is necessary
to include the data I have: Is a large deficiency with
similar breakpoints than Df(1)16-2-19 (described in
Flybase). The only difference being that the
latter is male sterile.
Df(1)17-25 :
Lethal with lethals ELC1, 25C4, 16-1-27, 16-3-98, 17-234
and LB11. Viable with Dfs. B-111, 16-3-95 and B-12 and
with l(1)AA-33. It is normal with 'bbl'and shows 'mal'
and 'mell' with Df(1)17-322. It shows 'mell' with
Df(1)16-3-35. It shows visible with 'ot'.
bk1: To the left of 'ot'. Left limit undetermined,
right from inclusion of 'ot'.
 bk2:Left  limit from inclusion of 'mell'; right from non-
inclusion of lethal AA-33.
In my previous mails I fail to clarify the following:
You have surely noted that for mapping all the
lethals many crosses appear as superfluous.
However, the information recovered is relevant when
mapping lethals induced by high LET radiations. In this
case, and due to the crowded ionization tracks generated
by neutrons it is necessary to clearly establish if the damage
inflicted is a continuous deficiency or it actually consists
of close double or multiple lethals in the same segment.
Thus it has a bearing on the reported RBE for recessive
lethals and the effect of ionization tracks generated by
high LET radiations in general ( that are so different from
those produced by ionization of lower LET like gamma or X rays).
I apologize for bothering you so much but
I thought it was relevant. Many thanks
Enzo.